Ritz U, Nusselt T, Sewing A, Ziebart T, Kaufmann K, Baranowski A, Rommens P M, Hofmann Alexander
Department of Orthopedics and Traumatology, University Medical Centre of the Johannes Gutenberg University, Langenbeckstr. 1, 55131, Mainz, Germany.
Biomet Deutschland GmbH, Berlin, Germany.
Clin Oral Investig. 2017 Jan;21(1):255-265. doi: 10.1007/s00784-016-1784-5. Epub 2016 Mar 12.
Targeted modifications of the bulk implant surfaces using bioactive agents provide a promising tool for improvement of the long-term bony and soft tissue integration of dental implants. In this study, we assessed the cellular responses of primary human gingival fibroblasts (HGF) to different surface modifications of titanium (Ti) and titanium nitride (TiN) alloys with type I collagen or cyclic-RGDfK-peptide in order to define a modification improving long-term implants in dental medicine.
Employing Ti and TiN implants, we compared the performance of simple dip coating and anodic immobilization of type I collagen that provided collagen layers of two different thicknesses. HGF were seeded on the different coated implants, and adhesion, proliferation, and gene expression were analyzed.
Although there were no strong differences in initial cell adhesion between the groups at 2 and 4 hours, we found that all surface modifications induced higher proliferation rates as compared to the unmodified controls. Consistently, gene expression levels of cell adhesion markers (focal adhesion kinase (FAK), integrin beta1, and vinculin), cell differentiation markers (FGFR1, TGFb-R1), extracellular protein markers (type I collagen, vimentin), and cytoskeletal protein marker aktinin-1 were consistently higher in all surface modification groups at two different time points of investigation as compared to the unmodified controls.
Our results indicate that simple dip coating of Ti and TiN with collagen is sufficient to induce in vitro cellular responses that are comparable to those of more reliable coating methods like anodic adsorption, chemical cross-linking, or RGD coating. TiN alloys do not possess any positive or adverse effects on HGF.
Our results demonstrate a simple, yet effective, method for collagen coating on titanium implants to improve the long term integration and stability of dental implants.
使用生物活性剂对植入体整体表面进行靶向修饰，为改善牙种植体的长期骨和软组织整合提供了一种有前景的工具。在本研究中，我们评估了原代人牙龈成纤维细胞（HGF）对钛（Ti）和氮化钛（TiN）合金用I型胶原蛋白或环RGDfK肽进行不同表面修饰的细胞反应，以确定一种可改善牙科医学中长期植入体的修饰方法。
使用Ti和TiN植入体，我们比较了简单浸涂和I型胶原蛋白阳极固定的性能，后者可提供两种不同厚度的胶原层。将HGF接种在不同涂层的植入体上，并分析细胞粘附、增殖和基因表达情况。
尽管在2小时和4小时时各实验组之间的初始细胞粘附没有显著差异，但我们发现与未修饰的对照组相比，所有表面修饰均诱导了更高的增殖率。一致地，在两个不同的研究时间点，所有表面修饰组中细胞粘附标记物（粘着斑激酶（FAK）、整合素β1和纽蛋白）、细胞分化标记物（FGFR1、TGFb-R1）、细胞外蛋白标记物（I型胶原蛋白、波形蛋白）和细胞骨架蛋白标记物辅肌动蛋白-1的基因表达水平均始终高于未修饰的对照组。
我们的结果表明，用胶原蛋白对Ti和TiN进行简单浸涂足以诱导体外细胞反应，这些反应与阳极吸附、化学交联或RGD涂层等更可靠的涂层方法所诱导的反应相当。TiN合金对HGF没有任何正面或负面影响。
我们的结果证明了一种在钛植入体上进行胶原蛋白涂层的简单而有效的方法，可改善牙种植体的长期整合和稳定性。
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