绒毛膜尿囊膜异种移植试验作为研究乳腺癌生物学特性的可靠模型

The Chorioallantoic Membrane Xenograft Assay as a Reliable Model for Investigating the Biology of Breast Cancer.

作者信息

Ranjan Raphela A, Muenzner Julienne K, Kunze Philipp, Geppert Carol I, Ruebner Matthias, Huebner Hanna, Fasching Peter A, Beckmann Matthias W, Bäuerle Tobias, Hartmann Arndt, Walther Wolfgang, Eckstein Markus, Erber Ramona, Schneider-Stock Regine

机构信息

Experimental Tumor Pathology, Institute of Pathology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.

Institute of Pathology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.

出版信息

Cancers (Basel). 2023 Mar 10;15(6):1704. doi: 10.3390/cancers15061704.

DOI:10.3390/cancers15061704

PMID:36980588

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10046776/

Abstract

The chorioallantoic membrane (CAM) assay is an alternative in vivo model that allows for minimally invasive research of cancer biology. Using the CAM assay, we investigated phenotypical and functional characteristics (tumor grade, mitosis rate, tumor budding, hormone receptor (HR) and HER2 status, Ki-67 proliferation index) of two breast cancer cell lines, MCF-7 and MDA-MB-231, which resemble the HR+ (luminal) and triple-negative breast cancer (TNBC) subgroups, respectively. Moreover, the CAM results were directly compared with murine MCF-7- and MDA-MB-231-derived xenografts and human patient TNBC tissue. Known phenotypical and biological features of the aggressive triple-negative breast cancer cell line (MDA-MB-231) were confirmed in the CAM assay, and mouse xenografts. Furthermore, the histomorphological and immunohistochemical variables assessed in the CAM model were similar to those in human patient tumor tissue. Given the confirmation of the classical biological and growth properties of breast cancer cell lines in the CAM model, we suggest this in vivo model to be a reliable alternative test system for breast cancer research to reduce murine animal experiments.

摘要

绒毛尿囊膜（CAM）试验是一种替代性体内模型，可用于癌症生物学的微创研究。我们利用CAM试验研究了两种乳腺癌细胞系MCF-7和MDA-MB-231的表型和功能特征（肿瘤分级、有丝分裂率、肿瘤芽生、激素受体（HR）和HER2状态、Ki-67增殖指数），这两种细胞系分别类似于HR阳性（管腔型）和三阴性乳腺癌（TNBC）亚组。此外，将CAM试验结果与源自小鼠MCF-7和MDA-MB-231的异种移植瘤以及人类患者TNBC组织进行了直接比较。侵袭性三阴性乳腺癌细胞系（MDA-MB-231）的已知表型和生物学特征在CAM试验以及小鼠异种移植瘤中得到了证实。此外，在CAM模型中评估的组织形态学和免疫组化变量与人类患者肿瘤组织中的相似。鉴于在CAM模型中证实了乳腺癌细胞系的经典生物学和生长特性，我们建议这种体内模型作为乳腺癌研究的可靠替代试验系统，以减少小鼠动物实验。