State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Sericulture, Textile and Biomass Science, Southwest University, Chongqing 400715, China.
WESTA College, Southwest University, Chongqing 400715, China.
Genes (Basel). 2023 Mar 4;14(3):649. doi: 10.3390/genes14030649.
Mutation rate is a crucial parameter in evolutionary genetics. However, the mutation rate of most species as well as the extent to which the environment can alter the genome of multicellular organisms remain poorly understood. Here, we used parents-progeny sequencing to investigate the mutation rate and spectrum of the domestic silkworm () among normal and two temperature stress conditions (32 °C and 0 °C). The rate of single-nucleotide mutations in the normal temperature rearing condition was 0.41 × 10 (95% confidence interval, 0.33 × 10-0.49 × 10) per site per generation, which was up to 1.5-fold higher than in four previously studied insects. Moreover, the mutation rates of the silkworm under the stresses are significantly higher than in normal conditions. Furthermore, the mutation rate varies less in gene regions under normal and temperature stresses. Together, these findings expand the known diversity of the mutation rate among eukaryotes but also have implications for evolutionary analysis that assumes a constant mutation rate among species and environments.
突变率是进化遗传学中的一个关键参数。然而，大多数物种的突变率以及环境在多大程度上可以改变多细胞生物的基因组仍然知之甚少。在这里，我们使用父母-后代测序来研究家蚕（）在正常和两种温度应激条件（32°C 和 0°C）下的突变率和突变谱。在正常温度饲养条件下，单核苷酸突变率为 0.41×10（95%置信区间，0.33×10-0.49×10）/位点/代，比之前研究的四种昆虫高 1.5 倍。此外，在应激条件下家蚕的突变率明显高于正常条件。此外，正常和温度应激下基因区域的突变率变化较小。总之，这些发现扩展了真核生物突变率的已知多样性，但也对进化分析产生了影响，因为进化分析假设物种和环境之间的突变率是恒定的。
