滑动电弧等离子体射流对自然污染大麦籽粒中霉菌区系和脱氧雪腐镰刀菌烯醇含量的影响。

Effect of Gliding Arc Plasma Jet on the Mycobiota and Deoxynivalenol Levels in Naturally Contaminated Barley Grains.

机构信息

Laboratory of Plasmas and Applications, Department of Physics, Faculty of Engineering and Sciences, São Paulo State University (UNESP), Guaratinguetá 12516-410, Brazil.

Laboratório de Microbiologia de Alimentos I, Departmento de Alimentos e Nutrição, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas-UNICAMP, Campinas 13083-862, Brazil.

出版信息

Int J Environ Res Public Health. 2023 Mar 14;20(6):5072. doi: 10.3390/ijerph20065072.

DOI:10.3390/ijerph20065072

PMID:36981981

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10049212/

Abstract

and are primary contaminants of barley, capable of producing several mycotoxins, mainly type B trichothecenes and zearalenone. Cold plasma decontamination has been gaining prominence, seeking to control the fungal and mycotoxin contamination of food and feed and to improve product quality. To reach this objective, the present study was divided into two parts. In the first part, and strains were exposed to gliding arc plasma jet (GAPJ). Cell viability tests showed the inactivation of after 15-min treatment, whereas showed to be resistant. In the second part, barley grains were treated by GAPJ for 10, 20, and 30 min, demonstrating a reduction of about 2 log CFU/g of the barley's mycobiota, composed of yeasts, strains belonging to the species complex, , and . A decrease in DON levels (up to 89%) was observed after exposure for 20 min. However, an increase in the toxin Deoxynivalenol-3-glucoside (D3G) was observed in barley grains, indicating a conversion of DON to D3G.

摘要

和是大麦的主要污染物，能够产生几种霉菌毒素，主要是 B 型单端孢霉烯族化合物和玉米赤霉烯酮。冷等离子体净化技术越来越受到关注，旨在控制食品和饲料中的真菌和霉菌毒素污染，并提高产品质量。为了实现这一目标，本研究分为两部分。在第一部分中，和菌株暴露于滑动弧等离子体射流（GAPJ）中。细胞活力测试表明，经过 15 分钟的处理后，被灭活，而表现出抗性。在第二部分中，大麦粒用 GAPJ 处理 10、20 和 30 分钟，使由酵母、种复合体、、和组成的大麦真菌菌群减少了约 2 个对数 CFU/g。暴露 20 分钟后，DON 水平下降了 89%。然而，在大麦粒中观察到毒素脱氧雪腐镰刀菌烯醇-3-葡萄糖苷（D3G）的增加，表明 DON 向 D3G 的转化。