Department of Internal Medicine, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu 01049-010, SP, Brazil.
Grupo Biologia y Control de Enfermedades Infeciosas (BCEI), Facultad de Ciencias exactas y Naturales, Universidad de Antioquia (UdeA), Medellín 050010, Colombia.
Int J Mol Sci. 2023 Mar 8;24(6):5212. doi: 10.3390/ijms24065212.
Dogs can be excellent models for spontaneous studies about breast cancers, presenting similarities in clinical behavior and molecular pathways of the disease. Thus, analyses of the canine transcriptome can identify deregulated genes and pathways, contributing to the identification of biomarkers and new therapeutic targets, benefiting humans and animals. In this context, this study aimed to determine the transcriptional profile of canine mammary ductal carcinoma and contribute to the clarification of the importance of deregulated molecules in the molecular pathways involved in the disease. Therefore, we used mammary ductal carcinoma tissue samples and non-tumor mammary tissue from the radical mastectomy of six female dogs. Sequencing was performed on the NextSeq-500 System platform. A comparison of carcinoma tissue and normal tissue revealed 633 downregulated and 573 upregulated genes, which were able to differentiate the groups by principal component analysis. Gene ontology analysis indicated that inflammatory, cell differentiation and adhesion, and extracellular matrix maintenance pathways were mainly deregulated in this series. The main differentially expressed genes observed in this research can indicate greater disease aggressiveness and worse prognosis. Finally, the study of the canine transcriptome indicates that it is an excellent model to generate information relevant to oncology in both species.
狗可以成为自发性乳腺癌研究的优秀模型，在疾病的临床行为和分子途径方面具有相似性。因此，对犬转录组的分析可以识别失调的基因和途径，有助于确定生物标志物和新的治疗靶点，使人类和动物受益。在这种情况下，本研究旨在确定犬乳腺导管癌的转录谱，并有助于阐明疾病相关分子途径中失调分子的重要性。为此，我们使用了来自 6 只雌性狗根治性乳房切除术的乳腺导管癌组织样本和非肿瘤乳腺组织。在 NextSeq-500 系统平台上进行了测序。癌组织与正常组织的比较显示，有 633 个下调基因和 573 个上调基因，主成分分析能够区分两组。基因本体分析表明，在该系列中，炎症、细胞分化和黏附以及细胞外基质维持途径主要失调。本研究中观察到的主要差异表达基因可能表明疾病侵袭性更强，预后更差。最后，犬转录组的研究表明，它是一种生成两种物种肿瘤学相关信息的优秀模型。
