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过表达一个 1R-MYB 转录因子基因()增加了 的耐盐和耐寒性。

Overexpression of a 1R-MYB Transcription Factor Gene () Increases Salt and Cold Tolerance in .

机构信息

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.

Key Laboratory of Horticultural Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China.

出版信息

Int J Mol Sci. 2023 Mar 9;24(6):5261. doi: 10.3390/ijms24065261.

Abstract

The MYB (v-MYB avian myeloblastosis viral oncogene homolog) transcription factor (TF) family has numerous members with complex and diverse functions, which play an indispensable role in regulating the response of plants to stress. In this study, a new 1R-MYB TF gene was obtained from (a diploid strawberry) by cloning technology and given a new name, . According to the subcellular localization results, FvMYB114 protein was a nuclear localization protein. Overexpression of greatly enhanced the adaptability and tolerance of to salt and low temperature. Under salt and cold stress, the transgenic plants had greater proline and chlorophyll contents and higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than the wild-type (WT) and unloaded-line (UL) . However, malondialdehyde (MDA) was higher in the WT and UL lines. These results suggested that may be involved in regulating the response of to salt stress and cold stress. can also promote the expression of genes, such as the genes , and related to salt stress and the genes , and related to cold stress, further improving the tolerance of transgenic plants to salt and cold stress.

摘要

MYB(v-MYB 禽成髓细胞瘤病毒癌基因同源物)转录因子(TF)家族拥有众多成员,具有复杂多样的功能,在调节植物对胁迫的反应中起着不可或缺的作用。本研究采用克隆技术从 (二倍体草莓)中获得了一个新的 1R-MYB TF 基因,并将其命名为 。根据亚细胞定位结果,FvMYB114 蛋白是一种核定位蛋白。过表达 可显著提高 对盐和低温的适应能力和耐受性。在盐和低温胁迫下,转基因植株的脯氨酸和叶绿素含量较高,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性也高于野生型(WT)和空载线(UL) 。然而,WT 和 UL 系中的丙二醛(MDA)含量较高。这些结果表明 可能参与调节 对盐胁迫和冷胁迫的反应。 还可以促进与盐胁迫相关的基因 、 、 以及与冷胁迫相关的基因 、 、 的表达,进一步提高转基因植物对盐和冷胁迫的耐受性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7abe/10048884/a80b9c874e05/ijms-24-05261-g001.jpg

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