Liu Wanda, Wang Tianhe, Wang Yu, Liang Xiaoqi, Han Jilong, Han Deguo
Horticulture Branch, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions/College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin, China.
Front Plant Sci. 2023 Feb 16;14:1141446. doi: 10.3389/fpls.2023.1141446. eCollection 2023.
Cold and drought stress considerably suppress the development of plants. In this study, a new MYB (v-myb avian myeloblastosis viral)TF gene, , was isolated from the and located in nucleus. has a positive response to low temperature and drought stress. After being introduced into , the physiological indicators of transgenic had corresponding changes under these two stresses, the activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) increased, electrolyte leakage rate (EL) and the content of proline increased, but the content of chlorophyll decreased. In addition, its overexpression can also activate the downstream expression of , , and related to cold stress and , , and related to drought stress. Based on these results, we speculate that can respond to cold and hydropenia signals, and can be used in transgenic technology to improve plant tolerance to low temperature and drought stress.
低温和干旱胁迫严重抑制植物的生长发育。在本研究中,从[具体植物名称]中分离出一个新的MYB(v-myb禽成髓细胞瘤病毒)转录因子基因[基因名称],其定位于细胞核。[基因名称]对低温和干旱胁迫有积极响应。将其导入[受体植物名称]后,转基因[受体植物名称]在这两种胁迫下的生理指标发生了相应变化,过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性增加,电解质渗漏率(EL)和脯氨酸含量增加,但叶绿素含量降低。此外,其过表达还能激活与冷胁迫相关的[基因名称1]、[基因名称2]、[基因名称3]以及与干旱胁迫相关的[基因名称4]、[基因名称5]、[基因名称6]的下游表达。基于这些结果,我们推测[基因名称]可以响应低温和缺水信号,并可用于转基因技术以提高植物对低温和干旱胁迫的耐受性。