哺乳动物细胞 DNA 损伤定量测量标准。
Standards for Quantitative Measurement of DNA Damage in Mammalian Cells.
机构信息
Materials Measurement Laboratory, National Institute of Standards and Technology, Biosystems and Biomaterials Division, Gaithersburg, MD 20899, USA.
出版信息
Int J Mol Sci. 2023 Mar 12;24(6):5427. doi: 10.3390/ijms24065427.
As the potential applications of DNA diagnostics continue to expand, there is a need for improved methods and standards for DNA analysis. This report describes several methods that could be considered for the production of reference materials for the quantitative measurement of DNA damage in mammalian cells. With the focus on DNA strand breaks, potentially useful methods for assessing DNA damage in mammalian cells are reviewed. The advantages and limitations of each method, as well as additional concerns with respect to reference material development, are also discussed. In conclusion, we outline strategies for developing candidate DNA damage reference materials that could be adopted by research laboratories in a wide variety of applications.
随着 DNA 诊断的潜在应用不断扩大，需要改进 DNA 分析的方法和标准。本报告描述了几种可用于生产哺乳动物细胞中 DNA 损伤定量测量参考物质的方法。本文重点介绍了 DNA 链断裂，综述了评估哺乳动物细胞中 DNA 损伤的潜在有用方法。讨论了每种方法的优缺点，以及在参考物质开发方面的其他问题。总之，我们概述了制定候选 DNA 损伤参考物质的策略，这些策略可以被广泛应用于各种研究实验室。
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