Yang Zhengmei, Dong Tingting, Dai Xibin, Wei Yiliang, Fang Yujie, Zhang Lei, Zhu Mingku, Nawaz Ghazala, Cao Qinghe, Xu Tao
Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics School of Life Sciences, Jiangsu Normal University, Xuzhou, China.
Department of Applied Biology, Chonnam National University, Gwangju, South Korea.
Front Plant Sci. 2022 Jul 7;13:879819. doi: 10.3389/fpls.2022.879819. eCollection 2022.
Sweetpotato [ (L.) Lam.] is an important food, vegetable and economic crop, but its productivity is remarkably affected by soil salinity. MiRNAs are a class of endogenous non-coding small RNAs that play an important role in plant resistance to salt stress. However, the function of miRNAs still remains largely unknown in sweetpotato under salt stress. Previously, we identified salt-responsive miRNAs in one salt-sensitive sweetpotato cultivar "Xushu 32." In this study, we identified miRNAs in another salt-tolerant cultivar "Xushu 22" by high-throughput deep sequencing and compared the salt-responsive miRNAs between these two cultivars with different salt sensitivity. We identified 687 miRNAs in "Xushu 22," including 514 known miRNAs and 173 novel miRNAs. Among the 759 miRNAs from the two cultivars, 72 and 109 miRNAs were specifically expressed in "Xushu 32" and "Xushu 22," respectively, and 578 miRNAs were co-expressed. The comparison of "Xushu 32" and "Xushu 22" genotypes showed a total of 235 miRNAs with obvious differential expression and 177 salt-responsive miRNAs that were obviously differently expressed between "Xushu 32" and "Xushu 22" under salt stress. The target genes of the miRNAs were predicted and identified using the Target Finder tool and degradome sequencing. The results showed that most of the targets were transcription factors and proteins related to metabolism and stress response. Gene Ontology analysis revealed that these target genes are involved in key pathways related to salt stress response and secondary redox metabolism. The comparative analysis of salt-responsive miRNAs in sweetpotato cultivars with different salt sensitivity is helpful for understanding the regulatory pattern of miRNA in different sweetpotato genotypes and improving the agronomic traits of sweetpotato by miRNA manipulation in the future.
甘薯[ (L.) Lam.]是一种重要的粮食、蔬菜和经济作物，但其生产力受到土壤盐分的显著影响。微小RNA（miRNA）是一类内源性非编码小RNA，在植物抗盐胁迫中发挥重要作用。然而，在盐胁迫下甘薯中miRNA的功能仍 largely未知。此前，我们在一个盐敏感型甘薯品种“徐薯32”中鉴定出了盐响应miRNA。在本研究中，我们通过高通量深度测序在另一个耐盐品种“徐薯22”中鉴定出miRNA，并比较了这两个具有不同盐敏感性的品种之间的盐响应miRNA。我们在“徐薯22”中鉴定出687个miRNA，包括514个已知miRNA和173个新miRNA。在这两个品种的759个miRNA中，分别有72个和109个miRNA在“徐薯32”和“徐薯22”中特异性表达，578个miRNA共表达。“徐薯32”和“徐薯22”基因型的比较显示，共有235个miRNA具有明显的差异表达，177个盐响应miRNA在盐胁迫下“徐薯32”和“徐薯22”之间明显差异表达。使用Target Finder工具和降解组测序对miRNA的靶基因进行了预测和鉴定。结果表明，大多数靶标是转录因子以及与代谢和应激反应相关的蛋白质。基因本体分析表明，这些靶基因参与了与盐胁迫反应和次生氧化还原代谢相关的关键途径。对不同盐敏感性甘薯品种中盐响应miRNA的比较分析有助于理解miRNA在不同甘薯基因型中的调控模式，并在未来通过miRNA操作改善甘薯的农艺性状。
