Valeri Andrea, Chiricosta Luigi, D'Angiolini Simone, Pollastro Federica, Salamone Stefano, Mazzon Emanuela
IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy.
Department of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, Italy.
Life (Basel). 2023 Mar 9;13(3):742. doi: 10.3390/life13030742.
Phytocannabinoids, with their variety of beneficial effects, represent a valid group of substances that could be employed as neurogenesis-enhancers or neuronal differentiation inducers. We focused our attention on the neuronal-related potential of cannabichromene (CBC) when administered to undifferentiated NSC-34 for 24 h. Transcriptomic analysis showed an upregulation of several neuronal markers, such as and , as well as indicators of neuronal differentiation process progression, such as . An in-depth investigation of the processes involved in neuronal differentiation indicates positive cytoskeleton remodeling by upregulation of and , and active differentiation-targeted transcriptional program, suggested by and After 48 h of treatment, the markers previously examined in the transcriptomic analysis are still overexpressed, like and , indicating that the differentiation process is still in progress. The lack of GFAP protein suggests that no astroglial differentiation is taking place, and it is reasonable to indicate the neuronal one as the ongoing one. These results indicate CBC as a potential neuronal differentiation inducer for NSC-34 cells.
植物大麻素具有多种有益作用，是一类有效的物质，可用作神经发生增强剂或神经元分化诱导剂。我们将注意力集中在将大麻色烯（CBC）给予未分化的NSC-34细胞24小时后其与神经元相关的潜力上。转录组分析显示，几种神经元标志物如[具体标志物1]和[具体标志物2]上调，以及神经元分化过程进展的指标如[具体指标]上调。对神经元分化所涉及过程的深入研究表明，通过[具体基因1]和[具体基因2]的上调实现了阳性细胞骨架重塑，以及由[具体基因3]和[具体基因4]表明的活跃的靶向分化转录程序。处理48小时后，转录组分析中先前检测的标志物如[具体标志物1]和[具体标志物2]仍过度表达，表明分化过程仍在进行。GFAP蛋白的缺乏表明没有发生星形胶质细胞分化，因此合理地表明正在进行的是神经元分化。这些结果表明CBC是NSC-34细胞潜在的神经元分化诱导剂。
