Kråkström Matilda, Dickens Alex M, Alves Marina Amaral, Forssten Sofia D, Ouwehand Arthur C, Hyötyläinen Tuulia, Orešič Matej, Lamichhane Santosh
Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland.
Global Health and Nutrition Sciences, International Flavors & Fragrances, 02460 Kantvik, Finland.
Metabolites. 2023 Feb 27;13(3):355. doi: 10.3390/metabo13030355.
Current evidence suggests that gut microbiome-derived lipids play a crucial role in the regulation of host lipid metabolism. However, not much is known about the dynamics of gut microbial lipids within the distinct gut biogeographic. Here we applied targeted and untargeted lipidomics to in vitro-derived feces. Simulated intestinal chyme was collected from in vitro gut vessels (V1-V4), representing proximal to distal parts of the colon after 24 and 48 h with/without polydextrose treatment. In total, 44 simulated chyme samples were collected from the in vitro colon simulator. Factor analysis showed that vessel and time had the strongest impact on the simulated intestinal chyme lipid profiles. We found that levels of phosphatidylcholines, sphingomyelins, triacylglycerols, and endocannabinoids were altered in at least one vessel (V1-V4) during simulation. We also found that concentrations of triacylglycerols, diacylglycerols, and endocannabinoids changed with time (24 vs. 48 h of simulation). Together, we found that the simulated intestinal chyme revealed a wide range of lipids that remained altered in different compartments of the human colon model over time.
目前的证据表明，肠道微生物群衍生的脂质在宿主脂质代谢的调节中起着关键作用。然而，对于不同肠道生物地理学区域内肠道微生物脂质的动态变化，我们了解得并不多。在这里，我们将靶向和非靶向脂质组学应用于体外培养的粪便。模拟肠内容物从体外肠道血管（V1-V4）收集，分别代表结肠近端至远端部分，在有/无聚葡萄糖处理的情况下培养24小时和48小时后进行收集。总共从体外结肠模拟器中收集了44个模拟肠内容物样本。因子分析表明，血管和时间对模拟肠内容物脂质谱的影响最大。我们发现，在模拟过程中，至少在一个血管（V1-V4）中，磷脂酰胆碱、鞘磷脂、三酰甘油和内源性大麻素的水平发生了变化。我们还发现，三酰甘油、二酰甘油和内源性大麻素的浓度随时间（模拟24小时与48小时）而变化。总之，我们发现模拟肠内容物揭示了广泛的脂质，这些脂质在人类结肠模型的不同隔室中随时间而持续变化。
