Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Centre for Synthetic Biology (CSB), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Molecules. 2021 Dec 23;27(1):73. doi: 10.3390/molecules27010073.
The intestinal absorption of dietary catechins is quite low, resulting in most of them being metabolized by gut microbiota in the colon. It has been hypothesized that microbiota-derived metabolites may be partly responsible for the association between catechin consumption and beneficial cardiometabolic effects. Given the profound differences in gut microbiota composition and microbial load between individuals and across different colon regions, this study examined how microbial (+)-catechin metabolite profiles differ between colon regions and individuals. Batch exploration of the interindividual variability in (+)-catechin microbial metabolism resulted in a stratification based on metabolic efficiency: from the 12 tested donor microbiota, we identified a fast- and a slow-converting microbiota that was subsequently inoculated to SHIME, a dynamic model of the human gut. Monitoring of microbial (+)-catechin metabolites from proximal and distal colon compartments with UHPLC-MS and UPLC-IMS-Q-TOF-MS revealed profound donor-dependent and colon-region-dependent metabolite profiles with 5-(3',4'-dihydroxyphenyl)-γ-valerolactone being the largest contributor to differences between the fast- and slow-converting microbiota and the distal colon being a more important region for (+)-catechin metabolism than the proximal colon. Our findings may contribute to further understanding the role of the gut microbiota as a determinant of interindividual variation in pharmacokinetics upon (+)-catechin ingestion.
膳食儿茶素的肠道吸收率相当低，导致其中大部分在结肠中被肠道微生物群代谢。有人假设，微生物衍生的代谢物可能部分解释了儿茶素的摄入与有益的心血管代谢效应之间的关联。鉴于个体之间和不同结肠区域之间的肠道微生物群组成和微生物负荷存在显著差异，本研究探讨了微生物 (+)-儿茶素代谢产物谱在结肠区域和个体之间的差异。对 (+)-儿茶素微生物代谢的个体间可变性进行批量探索，导致基于代谢效率的分层：从 12 种测试供体微生物群中，我们确定了一种快速转化和一种缓慢转化的微生物群，随后将其接种到 SHIME 中，这是人类肠道的动态模型。使用 UHPLC-MS 和 UPLC-IMS-Q-TOF-MS 监测来自近端和远端结肠腔室的微生物 (+)-儿茶素代谢物，揭示了深刻的供体依赖性和结肠区域依赖性代谢产物谱，其中 5-(3',4'-二羟基苯基)-γ-戊内酯是快速转化和缓慢转化微生物群之间差异的最大贡献者，远端结肠是 (+)-儿茶素代谢比近端结肠更为重要的区域。我们的发现可能有助于进一步了解肠道微生物群作为 (+)-儿茶素摄入后个体间药代动力学变异性决定因素的作用。
