Gill Harman, Sykes Ellen M E, Kumar Ayush, Sorensen John L
Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Microorganisms. 2023 Feb 26;11(3):590. doi: 10.3390/microorganisms11030590.
Fungi produce numerous secondary metabolites with intriguing biological properties for the health, industrial, and agricultural sectors. Herein, we report the high-yield isolation of phenolic natural products, N-formyl-4-hydroxyphenyl-acetamide (117 mg/L) and atraric acid (18 mg/L), from the ethyl acetate extract of the soil-derived fungus, . The structures of compounds and were elucidated through the detailed spectroscopic analysis of NMR and LCMS data. These compounds were assayed for their antimicrobial activities. It was observed that compounds and exhibited strong inhibition against a series of fungal strains but only weak antibacterial properties against multi-drug-resistant strains. More significantly, this is the first known instance of the isolation of atraric acid from a non-lichen fungal strain. We suggest the optimization of this fungal strain may exhibit elevated production of compounds and , potentially rendering it a valuable source for the industrial-scale production of these natural antimicrobial compounds. Further investigation is necessary to establish the veracity of this hypothesis.
真菌能产生大量具有有趣生物学特性的次生代谢产物，对健康、工业和农业领域都有重要意义。在此，我们报告了从土壤来源的真菌的乙酸乙酯提取物中高产分离出酚类天然产物N-甲酰基-4-羟基苯乙酰胺（约117毫克/升）和阿特拉酸（约18毫克/升）。通过对核磁共振（NMR）和液相色谱-质谱联用（LCMS）数据的详细光谱分析，阐明了化合物1和2的结构。对这些化合物的抗菌活性进行了测定。结果发现，化合物1和2对一系列真菌菌株表现出强烈抑制作用，但对多重耐药菌株的抗菌性能较弱。更重要的是，这是首次从非地衣真菌菌株中分离出阿特拉酸。我们认为，对该真菌菌株进行优化可能会提高化合物1和2的产量，使其有可能成为这些天然抗菌化合物工业规模生产的宝贵来源。有必要进行进一步研究以证实这一假设的真实性。
