Dougnon Victorien Tamègnon, Sintondji Kevin, Koudokpon Charles Hornel, Houéto Morènikè, Agbankpé Alidehou Jerrold, Assogba Phénix, Oussou Alida, Gnamy Anderson, Legba Boris, Idrissou Abdoulaye, Bankole Honoré Sourou
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou BP 526, Benin.
Microorganisms. 2023 Feb 28;11(3):617. doi: 10.3390/microorganisms11030617.
The use of catheters and bladder catheters in hospitals can increase the risk of bacterial infections. This study aimed to identify the bacterial strains involved in catheter-related infections (CRI) in southern Benin hospitals. The study included 407 samples, including 95 catheter tip samples and 312 urine samples collected from bladder catheters from patients on the first day and 48 h after admission. The catheter tip samples were analyzed using traditional bacterial isolation and identification methods, while the urine samples were analyzed using VITEK-2. Antibiotic sensitivity was tested using the Kirby Bauer method, and virulence and resistance genes were detected through standard PCR. The results showed a predominance of (53.5%), (23.3%), and (7.0%) among Gram-negative bacilli, and coagulase-negative as the most identified cocci. Bacterial susceptibility to antibiotics showed variable levels of resistance, with being detected in 42.9% of identified bacterial species, followed by SHV (26.2%) and (16.7%). The gene was only found in three identified bacterial strains, while A and B genes were detected in 3.2% of strains with a prevalence of 55% for the A gene. A prevalence of 18.8% for H was noted for the virulence genes. In conclusion, this study highlights the importance of following proper hygiene and aseptic practices during catheterization to effectively prevent CRIs. These findings should be used to improve interventions in hospitals and reduce healthcare-associated infections in developing countries.
医院中使用导管和膀胱导管会增加细菌感染的风险。本研究旨在确定贝宁南部医院与导管相关感染（CRI）有关的细菌菌株。该研究纳入了407份样本，包括95份导管尖端样本和312份从患者入院第一天及入院后48小时收集的膀胱导管尿液样本。导管尖端样本采用传统细菌分离和鉴定方法进行分析，而尿液样本则采用VITEK-2进行分析。使用 Kirby Bauer 方法测试抗生素敏感性，并通过标准PCR检测毒力和耐药基因。结果显示，革兰氏阴性杆菌中以（53.5%）、（23.3%）和（7.0%）为主，凝固酶阴性作为最常见的球菌。细菌对抗生素的敏感性显示出不同程度的耐药性，在所鉴定的细菌种类中，42.9%检测到，其次是SHV（26.2%）和（16.7%）。基因仅在三种鉴定出的细菌菌株中发现，而A和B基因在3.2%的菌株中检测到，A基因的流行率为55%。毒力基因H的流行率为18.8%。总之，本研究强调了在插管过程中遵循适当的卫生和无菌操作以有效预防CRI的重要性。这些发现应用于改善医院的干预措施，并减少发展中国家与医疗保健相关的感染。
