Caunedo-Jiménez María, Fernández-Colomer Belén, Fernández-Suárez Jonathan, Arias-Llorente Rosa Patricia, Lareu-Vidal Sonia, Mantecón-Fernández Laura, Solís-Sánchez Gonzalo, Suárez-Rodríguez Marta
Division of Neonatology, Department of Pediatrics, Central University Hospital of Asturias, Av. Roma s/n, E-33011 Oviedo, Spain.
Department of Microbiology, Central University Hospital of Asturias and Instituto de Investigación Sanitaria del Principado de Asturias, E-33011 Oviedo, Spain.
Microorganisms. 2023 Mar 12;11(3):732. doi: 10.3390/microorganisms11030732.
This prospective single-center study was designed to assess the clinical utility of the FilmArray blood culture identification (BCID) panel for improving the diagnostic accuracy in neonatal sepsis. Results obtained using the FilmArray BCID panel were correlated with results of blood culture in all consecutive neonates with suspicion of early-onset (EOS) and late-onset sepsis (LOS) attended in our service over a two-year period. A total of 102 blood cultures from 92 neonates were included, 69 (67.5%) in cases of EOS and 33 (32.3%) in LOS. The FilmArray BCID panel was performed in negative culture bottles at a median of 10 h of blood culture incubation (IQR 8-20), without differences by the type of sepsis. The FilmArray BCID panel showed a 66.7% sensitivity, 100% specificity, 100% positive predictive value, and 95.7% negative predictive value. There were four false-negative cases, three of which were in neonates with LOS, and there was one case of in one neonate with EOS. We conclude that the use of the FilmArray BCID panel in negative blood cultures from neonates with clinical suspicion of sepsis is useful in decision-making of starting or early withdrawal of empirical antimicrobials because of the high specificity and negative predictive values of this assay.
这项前瞻性单中心研究旨在评估FilmArray血培养鉴定（BCID）检测板在提高新生儿败血症诊断准确性方面的临床效用。在两年时间里，我们对所有连续就诊且疑似早发型（EOS）和晚发型败血症（LOS）的新生儿，将使用FilmArray BCID检测板获得的结果与血培养结果进行了相关性分析。共纳入了92例新生儿的102份血培养样本，其中EOS病例69份（67.5%），LOS病例33份（32.3%）。FilmArray BCID检测板在血培养孵育中位数为10小时（四分位间距8 - 20）时对阴性培养瓶进行检测，不同类型败血症之间无差异。FilmArray BCID检测板的敏感性为66.7%，特异性为100%，阳性预测值为100%，阴性预测值为95.7%。有4例假阴性病例，其中3例为LOS新生儿，1例为EOS新生儿。我们得出结论，对于临床怀疑败血症的新生儿，在阴性血培养中使用FilmArray BCID检测板，由于该检测具有高特异性和阴性预测值，有助于决定是否开始或提前停用经验性抗菌药物。
