Schneider György, Pásztor Dorina, Szabó Péter, Kőrösi László, Kishan Nandyala Siva, Raju Penmetsa Appala Rama Krishna, Calay Rajnish Kaur
Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, Hungary.
Department of Geology and Meteorology, Faculty of Sciences, University of Pécs, Ifjúság Str. 6, H-7624 Pécs, Hungary.
Microorganisms. 2023 Mar 17;11(3):781. doi: 10.3390/microorganisms11030781.
To develop efficient microbial fuel cell systems for green energy production using different waste products, establishing characterised bacterial consortia is necessary. In this study, bacteria with electrogenic potentials were isolated from mud samples and examined to determine biofilm-formation capacities and macromolecule degradation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identifications have revealed that isolates represented 18 known and 4 unknown genuses. They all had the capacities to reduce the Reactive Black 5 stain in the agar medium, and 48 of them were positive in the wolfram nanorod reduction assay. The isolates formed biofilm to different extents on the surfaces of both adhesive and non-adhesive 96-well polystyrene plates and glass. Scanning electron microscopy images revealed the different adhesion potentials of isolates to the surface of carbon tissue fibres. Eight of them (15%) were able to form massive amounts of biofilm in three days at 23 °C. A total of 70% of the isolates produced proteases, while lipase and amylase production was lower, at 38% and 27% respectively. All of the macromolecule-degrading enzymes were produced by 11 isolates, and two isolates of them had the capacity to form a strong biofilm on the carbon tissue one of the most used anodic materials in MFC systems. This study discusses the potential of the isolates for future MFC development applications.
为了利用不同的废弃物开发用于绿色能源生产的高效微生物燃料电池系统，建立具有特征的细菌群落是必要的。在本研究中，从泥浆样本中分离出具有产电潜力的细菌，并对其生物膜形成能力和大分子降解情况进行了检测。基质辅助激光解吸/电离飞行时间质谱鉴定表明，分离株代表18个已知属和4个未知属。它们都有在琼脂培养基中还原活性黑5染料的能力，其中48株在钨纳米棒还原试验中呈阳性。分离株在粘性和非粘性96孔聚苯乙烯板以及玻璃表面形成生物膜的程度不同。扫描电子显微镜图像显示了分离株对碳组织纤维表面的不同粘附潜力。其中8株（15%）能够在23℃下3天内形成大量生物膜。总共70%的分离株产生蛋白酶，而脂肪酶和淀粉酶的产生率较低，分别为38%和27%。所有的大分子降解酶都是由11株分离株产生的，其中两株能够在碳组织上形成强生物膜，碳组织是微生物燃料电池系统中最常用的阳极材料之一。本研究讨论了分离株在未来微生物燃料电池开发应用中的潜力。
