Faculdade de Engenharia Química, Instituto de Tecnologia, Universidade Federal do Pará, Rua Augusto Corrêa, 01, Guamá, Belem 66075-900, PA, Brazil.
Laboratório Adolpho Ducke, Coordenação de Botânica, Museu Paraense Emílio Goeldi, Av. Perimetral, 1901, Terra Firme, Belem 66077-830, PA, Brazil.
Molecules. 2023 Mar 14;28(6):2633. doi: 10.3390/molecules28062633.
The essential oils (EOs) of (Gsch) and (Xfru) (Annonaceae) were obtained by hydrodistillation, and their chemical composition was evaluated by gas chromatography-mass spectrometry (GC/MS). Herbicide activity was measured by analyzing the seed germination percentage and root and hypocotyl elongation of two invasive species: and . The highest yield was obtained for the EO of Xfru (1.06%). The chemical composition of Gsch was characterized by the presence of the oxygenated sesquiterpenes spathulenol (22.40%) and caryophyllene oxide (14.70%). Regarding the EO of Xfru, the hydrocarbon monoterpenes α-pinene (35.73%) and β-pinene (18.90%) were the components identified with the highest concentrations. The germination of seeds of . (13.33 ± 5.77%) showed higher resistance than that of seeds of . (86.67 ± 5.77%). was also more sensitive to the EO of Xfru in terms of radicle (55.22 ± 2.72%) and hypocotyl (71.12 ± 3.80%) elongation, while . showed greater sensitivity to the EO of Gsch. To screen the herbicidal activity, the molecular docking study of the major and potent compounds was performed against 4-hydroxyphenylpyruvate dioxygenase (HPPD) protein. Results showed good binding affinities and attributed the strongest inhibitory activity to δ-cadinene for the target protein. This work contributes to the study of the herbicidal properties of the EOs of species of Annonaceae from the Amazon region.
（Gsch）和（Xfru）（番荔枝科）的精油通过水蒸馏法获得，并通过气相色谱-质谱联用仪（GC/MS）评估其化学成分。通过分析两种入侵物种的种子发芽率和根和下胚轴伸长率来测量除草剂活性：和。Xfru 的精油产率最高（1.06%）。Gsch 的化学成分的特征在于含氧倍半萜 spathulenol（22.40%）和石竹烯氧化物（14.70%）的存在。关于 Xfru 的精油，单萜烃 α-蒎烯（35.73%）和β-蒎烯（18.90%）是鉴定浓度最高的成分。种子的发芽率。（13.33 ± 5.77%）显示出比种子更高的抗性。（86.67 ± 5.77%）。就根（55.22 ± 2.72%）和下胚轴（71.12 ± 3.80%）伸长而言，Xfru 的精油对也更敏感，而。对 Gsch 的精油更敏感。为了筛选除草剂活性，对主要和有效化合物对 4-羟基苯丙酮酸双加氧酶（HPPD）蛋白的分子对接研究进行了。结果显示出良好的结合亲和力，并归因于靶蛋白的最强抑制活性为δ-杜松烯。这项工作有助于研究来自亚马逊地区番荔枝科植物精油的除草特性。
