Migratory Wild Birds as Potential Long-Distance Transmitters of Infection.

is a worldwide distributed zoonotic protozoan capable of infecting a wide range of mammals (including humans) and birds as intermediate hosts. Migratory wild birds, through interconnecting countries along their flyways, can play a role in the spatial spread of and could contribute to its sylvatic cycle. Additionally, hunted wild birds used for meat consumption could represent a further source of human infection. To determine the presence of in wild birds, a total of 50 individuals belonging to the Anseriformes and Charadriiformes orders were sampled during the 2021-2022 hunting season in Northern Italy. Cardiac muscle samples of three Northern shovelers (, two wild mallards (), one Eurasian teal (), and one Northern lapwing () were positive for the molecular detection of based on a targeted amplification of the B1 gene. A 14% (7/50) overall positivity was observed in the sampled population. Results from this study suggest a moderate exposure of wild aquatic birds to , highlighting the importance of a further characterization of in its wildlife hosts.