Rosso Laura C, Romano Francesca, Pentimone Isabella, Ciancio Aurelio, Colagiero Mariantonietta
Consiglio Nazionale delle Ricerche, Istituto per la Protezione Sostenibile delle Piante, CNR, Via G. Amendola 122/D, 70126 Bari, Italy.
Medical Genetics, Department of Precision Medicine, Università degli Studi della Campania, via Luigi De Crecchio 7, Naples 80131, Naples, Italy.
Plants (Basel). 2023 Mar 11;12(6):1284. doi: 10.3390/plants12061284.
The expression of WRKY transcription factors and plant defense-related genes was studied in the roots of Apulian tomato () cv Regina di Fasano (accessions MRT and PLZ) endophytically colonized by and parasitized or not by the root-knot nematode (RKN) . The effect on plant growth, nematode parasitism and histological aspect of the interaction were considered. The association of with RKN-parasitized MRT plants increased the total biomass and shoot fresh weight in comparison with healthy plants and with those only parasitized by RKN, without the endophyte. However, the PLZ accession showed no significant difference in the observed biometric parameters. The number of RKN-induced galls per plant was not affected by endophytism eight days after inoculation. No histological changes were observed in the nematode feeding sites in the presence of the fungus. Gene expression analysis showed an accession-specific response to with differential activation of WRKY-related genes. No significant change was found for expression in nematode-parasitized plants compared with control roots, confirming cultivar susceptibility. Data indicate genotype-specific responses of the WRKY genes to parasitism examined in roots with nematodes and/or endophytic . At 25 days post-inoculation with , no significant difference was observed in the expression of defense-related genes in both accessions, suggesting that salicylic acid (SA) ( and ) and jasmonate (JA) related genes () are not active during endophytism.
研究了被[具体内生真菌名称未给出]内生定殖且被根结线虫（RKN）[具体线虫名称未给出]寄生或未寄生的阿普利亚番茄（品种为Regina di Fasano，种质编号MRT和PLZ）根系中WRKY转录因子和植物防御相关基因的表达情况。同时考虑了其对植物生长、线虫寄生以及相互作用组织学方面的影响。与健康植株以及仅被RKN寄生而无内生真菌的植株相比，[具体内生真菌名称未给出]与被RKN寄生的MRT植株的关联增加了总生物量和地上部鲜重。然而，PLZ种质在观察到的生物统计学参数方面没有显著差异。接种8天后，每株植物上RKN诱导的虫瘿数量不受内生菌感染的影响。在有真菌存在的情况下，线虫取食部位未观察到组织学变化。基因表达分析表明，对[具体内生真菌名称未给出]存在种质特异性反应，WRKY相关基因有不同程度的激活。与对照根相比，线虫寄生植物中[具体基因名称未给出]的表达没有显著变化，证实了品种的易感性。数据表明，在被线虫和/或[具体内生真菌名称未给出]寄生的根系中，WRKY基因存在基因型特异性反应。接种[具体内生真菌名称未给出]25天后，两个种质中防御相关基因的表达均未观察到显著差异，这表明水杨酸（SA）（[具体SA相关基因名称未给出]和[具体SA相关基因名称未给出]）和茉莉酸（JA）相关基因（[具体JA相关基因名称未给出]）在内生菌感染期间不活跃。
