用扩散盒技术研究小鼠卵黄囊造血作用。
Murine yolk sac hematopoiesis studied with the diffusion chamber technique.
作者信息
Symann M, Anckaert M A, Cordier A, Rodhain J, Sokal G
出版信息
Exp Hematol. 1978 Nov;6(10):749-59.
The proliferation and differentiation of visceral and of parietal yolk sac cells from 9-day-old mouse embryos were studied in diffusion chambers (DC). After culture, the visceral yolk sac yielded predominantly macrophages whereas the parietal yolk sac displayed mainly plasmocyte-like cells. It is shown the latter do not synthesize immunoglobulin but rather elaborate Reichert's membrane. Neither visceral nor parietal yolk sac contained detectable pluripotent stem cells (CFUs) prior to or after DC culture, a finding which is discussed in the light of the current literature.
在扩散小室（DC）中研究了9日龄小鼠胚胎的脏层和壁层卵黄囊细胞的增殖与分化。培养后，脏层卵黄囊主要产生巨噬细胞，而壁层卵黄囊主要呈现浆细胞样细胞。结果表明，后者不合成免疫球蛋白，而是形成赖歇特膜。在DC培养之前或之后，脏层和壁层卵黄囊中均未检测到多能干细胞（CFU），结合当前文献对这一发现进行了讨论。
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