Lončarević Andrea, Ostojić Karla, Urlić Inga, Rogina Anamarija
Faculty of Chemical Engineering and Technology, University of Zagreb, Trg Marka Marulića 19, HR-10000 Zagreb, Croatia.
Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia.
Polymers (Basel). 2023 Mar 16;15(6):1480. doi: 10.3390/polym15061480.
The aim of this work was to prepare bimetallic chitosan microgels with high sphericity and investigate the influences of metal-ion type and content on the size, morphology, swelling, degradation and biological properties of microgels. Amino and hydroxyl groups of chitosan (deacetylation degree, , of 83.2% and 96.9%) served as ligands in the Cu-Zn/chitosan complexes with various contents of cupric and zinc ions. The electrohydrodynamic atomization process was used to produce highly spherical microgels with a narrow size distribution and with surface morphology changing from wrinkled to smooth by increasing Cu ions' quantity in bimetallic systems for both used chitosans. The size of the bimetallic chitosan particles was estimated to be between 60 and 110 µm for both used chitosans, and FTIR spectroscopy indicated the formation of complexes through physical interactions between the chitosans' functional groups and metal ions. The swelling capacity of bimetallic chitosan particles decreases as the and copper (II) ion content increase as a result of stronger complexation with respect to zinc (II) ions. Bimetallic chitosan microgels showed good stability during four weeks of enzymatic degradation, and bimetallic systems with smaller amounts of Cu ions showed good cytocompatibility for both used chitosans.
本研究旨在制备具有高球形度的双金属壳聚糖微凝胶，并研究金属离子类型和含量对微凝胶的尺寸、形态、溶胀、降解及生物学性能的影响。壳聚糖（脱乙酰度分别为83.2%和96.9%）的氨基和羟基在含有不同含量铜离子和锌离子的Cu-Zn/壳聚糖络合物中作为配体。采用电流体动力学雾化法制备了尺寸分布窄、高度球形的微凝胶，对于两种壳聚糖，在双金属体系中通过增加铜离子的量，微凝胶的表面形态从皱缩变为光滑。两种壳聚糖的双金属壳聚糖颗粒尺寸估计在60至110 µm之间，傅里叶变换红外光谱表明壳聚糖官能团与金属离子通过物理相互作用形成了络合物。由于与锌（II）离子的络合作用更强，随着锌（II）和铜（II）离子含量的增加，双金属壳聚糖颗粒的溶胀能力降低。双金属壳聚糖微凝胶在四周的酶降解过程中表现出良好的稳定性，对于两种壳聚糖，含少量铜离子的双金属体系均表现出良好的细胞相容性。
