锌提高壳聚糖的抗菌、抗炎和细胞迁移活性，从而促进伤口愈合。

Zinc improves antibacterial, anti-inflammatory and cell motility activity of chitosan for wound healing applications.

机构信息

Centre for Functional and Surface Functionalized Glass, Alexander Dubček University of Trenčín, 911 50 Trenčín, Slovakia; Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.

Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.

出版信息

Int J Biol Macromol. 2022 Jul 31;213:845-857. doi: 10.1016/j.ijbiomac.2022.05.199. Epub 2022 Jun 3.

We report the successful preparation and characterization of chitosan-Zn complex (ChiZn) in the form of films, intended to enhance the biological performance of chitosan by the presence of Zn as antibacterial agent and biologically active ion. The influence of Zn chelation on morphology and structure of chitosan was assessed by scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy and infrared spectroscopy. The biodegradability study of ChiZn showed a sustained release of Zn up to 2 mg/mL. No toxic response was observed toward stromal cell line ST-2 in indirect contact with the ChiZn films. The dissolution product of ChiZn showed improved wound closure (88% closure) compared to the positive control group (70% closure). Moreover, ChiZn exhibited antibacterial activity against S. aureus together with a slight increase (~30%) in the secretion of VEGF and moderate decrease in nitric oxide evolution. Our findings indicate that ChiZn could be used as a safe and effective wound healing agent.

我们报告了壳聚糖-锌复合物（ChiZn）薄膜的成功制备和表征，旨在通过引入 Zn 作为抗菌剂和具有生物活性的离子来增强壳聚糖的生物学性能。扫描电子显微镜结合能谱和红外光谱评估了 Zn 螯合对壳聚糖形貌和结构的影响。ChiZn 的生物降解研究表明，Zn 持续释放高达 2 mg/mL。与 ChiZn 薄膜间接接触的基质细胞系 ST-2 未观察到毒性反应。与阳性对照组（70%闭合）相比，ChiZn 的溶解产物显示出更好的伤口闭合（88%闭合）。此外，ChiZn 对金黄色葡萄球菌表现出抗菌活性，同时 VEGF 的分泌略有增加（约 30%），一氧化氮的产生适度减少。我们的研究结果表明，ChiZn 可用作安全有效的伤口愈合剂。