Magdziarz Sylwia, Boguń Maciej, Frączyk Justyna
Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.
Łukasiewicz-Lodz Institute of Technology, Sklodowskiej-Curie 19/27, 90-570 Lodz, Poland.
Polymers (Basel). 2023 Mar 21;15(6):1551. doi: 10.3390/polym15061551.
The cross-linking of polysaccharides is a universal approach to affect their structure and physical properties. Both physical and chemical methods are used for this purpose. Although chemical cross-linking provides good thermal and mechanical stability for the final products, the compounds used as stabilizers can affect the integrity of the cross-linked substances or have toxic properties that limit the applicability of the final products. These risks might be mitigated by using physically cross-linked gels. In the present study, we attempted to obtain hybrid materials based on carbon nonwovens with a layer of cross-linked hyaluronan and peptides that are fragments of bone morphogenetic proteins (BMPs). A variety of cross-linking procedures and cross-linking agents (1,4-butanediamine, citric acid, and BDDE) were tested to find the most optimal method to coat the hydrophobic carbon nonwovens with a hydrophilic hyaluronic acid (HA) layer. Both the use of hyaluronic acid chemically modified with BMP fragments and a physical modification approach (layer-by-layer method) were proposed. The obtained hybrid materials were tested with the spectrometric (MALDI-TOF MS) and spectroscopic methods (IR and 1H-NMR). It was found that the chemical cross-linking of polysaccharides is an effective method for the deposition of a polar active substance on the surface of a hydrophobic carbon nonwoven fabric and that the final material is highly biocompatible.
多糖的交联是一种影响其结构和物理性质的通用方法。物理方法和化学方法都可用于此目的。尽管化学交联为最终产品提供了良好的热稳定性和机械稳定性，但用作稳定剂的化合物可能会影响交联物质的完整性或具有毒性，从而限制了最终产品的适用性。使用物理交联凝胶可能会减轻这些风险。在本研究中，我们试图获得基于碳无纺布的杂化材料，该材料带有一层交联透明质酸和作为骨形态发生蛋白（BMP）片段的肽。测试了各种交联程序和交联剂（1,4-丁二胺、柠檬酸和BDDE），以找到用亲水性透明质酸（HA）层包覆疏水性碳无纺布的最佳方法。我们提出了使用经BMP片段化学修饰的透明质酸以及物理修饰方法（逐层法）。用光谱法（基质辅助激光解吸电离飞行时间质谱）和光谱方法（红外光谱和1H-核磁共振）对所得杂化材料进行了测试。结果发现，多糖的化学交联是在疏水性碳无纺布表面沉积极性活性物质的有效方法，且最终材料具有高度生物相容性。
