Center for Bioscience & Biotechnology, University of Science, Ho Chi Minh City, Vietnam.
Vietnam National University, Ho Chi Minh City, Vietnam.
Biotechniques. 2023 Mar;74(3):123-129. doi: 10.2144/btn-2022-0083. Epub 2023 Mar 29.
is a critical host for producing recombinant proteins. However, the SDS-PAGE process, including the sample preparation steps, varies among -related studies, making it impossible to compare findings. Hence, this paper provides a simple guide to culture and collect cells through an OD measurement and a protocol for SDS-PAGE. These techniques were applied to check the expression of a BgaB, a reporter protein and LukF-PV, a potential vaccine candidate against , in the cytoplasm of under the control of a strong promoter, P. This protocol could be helpful for scientists in preparing samples and generating an SDS-PAGE experiment, as well as favoring the unification of research about protein expression in .
是生产重组蛋白的关键宿主。然而，SDS-PAGE 过程，包括样品制备步骤，在不同的 - 相关研究中有所不同，使得结果无法进行比较。因此，本文提供了一种通过 OD 测量培养和收集 细胞的简单指南，以及 SDS-PAGE 的方案。这些技术被应用于检查 BgaB、报告蛋白和 LukF-PV 在细胞质中的表达，BgaB 和 LukF-PV 是一种针对 的潜在疫苗候选物，在强启动子 P 的控制下在 中表达。该方案可以帮助科学家准备样品并进行 SDS-PAGE 实验，同时有利于蛋白质在 中表达的研究的统一。
