Preparation of cell samples and generation of an SDS-PAGE.

is a critical host for producing recombinant proteins. However, the SDS-PAGE process, including the sample preparation steps, varies among -related studies, making it impossible to compare findings. Hence, this paper provides a simple guide to culture and collect cells through an OD measurement and a protocol for SDS-PAGE. These techniques were applied to check the expression of a BgaB, a reporter protein and LukF-PV, a potential vaccine candidate against , in the cytoplasm of under the control of a strong promoter, P. This protocol could be helpful for scientists in preparing samples and generating an SDS-PAGE experiment, as well as favoring the unification of research about protein expression in .