Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail, Saudi Arabia.
Molecular Diagnostic & Personalized Therapeutic Unit, University of Hail, Hail, Saudi Arabia.
Biotechnol Genet Eng Rev. 2024 Nov;40(3):1709-1725. doi: 10.1080/02648725.2023.2196477. Epub 2023 Mar 29.
The antioxidant and antiglycation activities of the leaf extracts were evaluated using assays. The antioxidant activity was determined using the α, α-diphenyl-β-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assays. ferric reducing activity was evaluated using the ferric reducing antioxidant power assay. The antiglycation potential of the extract was evaluated using dinitrophenylhydrazine, thiobarbituric acid and protein thiol assays. The inhibition of the formation of advanced glycation end products (AGEs) was detected using AGE-specific fluorescence with a fluorescence spectrophotometer. This study was aimed at investigating the potential of Forssk. leaf extracts, which have abundant bioactive constituents, including polyphenols and flavonoids, in inhibiting glycation and cancer. The results show that the aqueous and methanolic leaf extracts are rich in phenolic and flavonoid compounds. Both extracts showed potent antioxidant activities. Furthermore, the methanolic extract showed antiglycation activities, as assessed using an model of bovine serum albumin glycation with D-ribose. The polyphenol- and flavonoid-rich leaf extracts exhibit antiglycation, chemopreventive and antioxidant activities and have potential for use in the treatment of diseases, such as cancer, which involve oxidative and glycative impairment of cellular proteins.
采用 测定法评估了叶提取物的抗氧化和抗糖化活性。采用 α,α-二苯基-β- picrylhydrazyl 和 2,2'-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)自由基清除测定法测定抗氧化活性。采用铁还原抗氧化能力测定法评估铁还原活性。采用二硝基苯肼、硫代巴比妥酸和蛋白质巯基测定法评估提取物的抗糖化潜力。使用荧光分光光度计检测与 AGE 特异性荧光检测抑制晚期糖基化终产物 (AGEs) 的形成。本研究旨在研究富含生物活性成分（包括多酚和类黄酮）的 Forssk. 叶提取物在抑制糖化和癌症方面的潜力。结果表明，水提和甲醇提 叶提取物富含酚类和类黄酮化合物。两种提取物均表现出很强的抗氧化活性。此外，甲醇提取物在牛血清白蛋白与 D-核糖糖化的 模型中表现出抗糖化活性。富含多酚和类黄酮的 叶提取物具有抗糖化、化学预防和抗氧化活性，有望用于治疗涉及细胞蛋白质氧化和糖化损伤的疾病，如癌症。
