Grzegorczyk-Karolak Izabela, Gołąb Krzysztof, Gburek Jakub, Wysokińska Halina, Matkowski Adam
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, ul. Muszynskiego 1, Lodz 90-151, Poland.
Department of Pharmaceutical Biochemistry, Wroclaw Medical University, ul. Borowska 211A, Wroclaw 50-556, Poland.
Molecules. 2016 Jun 14;21(6):739. doi: 10.3390/molecules21060739.
Methanolic extracts from the aerial parts and roots of two Scutellaria species, S. alpina and S. altissima, and five polyphenols from these plants demonstrated a significant ability to inhibit the formation of advanced glycation end-products (AGE) in vitro. S. alpina, which is richer in polyphenolic compounds, had strong antiglycation properties. These extracts demonstrated also high activity in the FRAP (ferric-reducing antioxidant power), antiradical (DPPH) and lipid peroxidation inhibition assays. Among the pure compounds, baicalin was the strongest glycation inhibitor (90.4% inhibition at 100 μg/mL), followed by luteolin (85.4%). Two other flavone glycosides had about half of this activity. Verbascoside was similar to the reference drug aminoguanidine (71.2% and 75.9%, respectively). The strong correlation observed between AGE inhibition and total flavonoid content indicated that flavonoids contribute significantly to antiglycation properties. A positive correlation was also observed between antiglycative and antioxidant activities. The studied skullcap species can be considered as a potential source of therapeutic agents for hyperglycemia-related disorders.
两种黄芩属植物高山黄芩(Scutellaria alpina)和高茎黄芩(Scutellaria altissima)地上部分及根部的甲醇提取物,以及这些植物中的五种多酚类物质,在体外均表现出显著抑制晚期糖基化终产物(AGE)形成的能力。富含多酚类化合物的高山黄芩具有较强的抗糖基化特性。这些提取物在FRAP(铁还原抗氧化能力)、抗自由基(DPPH)和脂质过氧化抑制试验中也表现出高活性。在纯化合物中,黄芩苷是最强的糖基化抑制剂(100μg/mL时抑制率为90.4%),其次是木犀草素(85.4%)。另外两种黄酮苷的活性约为上述两者的一半。毛蕊花糖苷与参比药物氨基胍的活性相似(分别为71.2%和75.9%)。AGE抑制与总黄酮含量之间的强相关性表明,黄酮类化合物对抗糖基化特性有显著贡献。抗糖基化活性与抗氧化活性之间也存在正相关。所研究的黄芩属植物可被视为高血糖相关疾病治疗药物的潜在来源。