Panferov Vasily G, Ivanov Nikita A, Brinc Davor, Fabros Anselmo, Krylov Sergey N
Department of Chemistry, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada.
A.N. Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences, 33 Leninsky Prospect, Moscow 119071, Russia.
ACS Sens. 2023 Apr 28;8(4):1792-1798. doi: 10.1021/acssensors.3c00130. Epub 2023 Mar 29.
Serological assays detect the presence of specific antibodies in blood. There are urgent and important applications for serological point-of-care (POC) assays. However, available detection methods are either insufficiently sensitive or too complex for POC settings. Here, we demonstrate that lateral flow immunoassay (LFIA), which is arguably the simplest universal molecular detection approach, can serve as a methodological platform for highly sensitive serological POC assays if combined with a simple, fast, and inexpensive electrophoretic step. In this work, we compared such electrophoretically driven LFIA (eLFIA) with conventional LFIA for the detection of immunoglobulins G against hepatitis B and C in serum. The limit of detection of eLFIA was proven to be 1000 times lower than that of conventional LFIA and sufficiently low to support clinical serological tests. eLFIA takes less than 10 min, requires only a minor accessory powered by a small 9 V battery, and can be performed by an untrained person in the POC environment using a 3 μL specimen of finger-prick capillary blood.
血清学检测可检测血液中特定抗体的存在。血清学即时检测（POC）有紧急且重要的应用。然而，现有的检测方法对于POC环境而言，要么灵敏度不足，要么过于复杂。在此，我们证明，侧向流动免疫分析（LFIA）可说是最简单的通用分子检测方法，若与一个简单、快速且廉价的电泳步骤相结合，它可以作为高灵敏度血清学POC检测的方法平台。在这项工作中，我们将这种电泳驱动的LFIA（eLFIA）与传统LFIA用于检测血清中抗乙型肝炎和丙型肝炎的免疫球蛋白G进行了比较。结果证明，eLFIA的检测限比传统LFIA低1000倍，且足够低以支持临床血清学检测。eLFIA耗时不到10分钟，仅需一个由小型9伏电池供电的小型配件，并且在POC环境中，未经培训的人员可以使用3微升指尖毛细血管血标本进行操作。
