Laboratory for Studies of Interactions Between Nutrition and Genetics, LEING, Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
J Nutr Biochem. 2023 Jul;117:109336. doi: 10.1016/j.jnutbio.2023.109336. Epub 2023 Mar 28.
This study aimed to evaluate the preventive and therapeutic effects of coffee consumption on molecular changes and adipose tissue remodeling in a murine model of high-fat diet-induced obesity. Three-month-old C57BL/6 mice were initially divided into three groups, namely, control (C), high-fat (HF), and coffee prevention (HF-CP) groups, and the HF group was subdivided at the end of the 10th week into two subgroups, an HF group and a coffee treatment (HF-CT) group; thus, a total of four groups were investigated at the 14th week of the experiment. The HF-CP group had lower body mass than the HF group (-7%, P < .05) and a better distribution of adipose tissue. Both groups that received coffee (HF-CP and HF-CT) showed improved glucose metabolism compared with the HF group. Coffee consumption also attenuated adipose tissue inflammation and showed decreased macrophage infiltration and lower IL-6 levels compared with the HF group (HF-CP: -337% %, P < .05; HF-CT: -275%, P < .05). Hepatic steatosis and inflammation were attenuated in the HF-CP and HF-CT groups. The HF-CP group showed more pronounced expression of genes involved in adaptive thermogenesis and mitochondrial biogenesis (PPARγ, Prdm16, Pcg1α, β3-adrenergic receptor, Ucp-1, and Opa-1) than the other experimental groups. Preventive coffee consumption associated with a high-fat diet ameliorates the metabolic profile related to the development of obesity and its comorbidities.
本研究旨在评估咖啡摄入对高脂肪饮食诱导肥胖小鼠模型中分子变化和脂肪组织重构的预防和治疗作用。将 3 月龄 C57BL/6 小鼠最初分为三组，即对照组（C）、高脂肪组（HF）和咖啡预防组（HF-CP），HF 组在第 10 周末再分为两组，即 HF 组和咖啡治疗组（HF-CT）；因此，在实验的第 14 周共研究了四组。与 HF 组相比，HF-CP 组的体重较低（-7%，P <.05），脂肪组织分布更好。与 HF 组相比，接受咖啡的两组（HF-CP 和 HF-CT）的葡萄糖代谢均有所改善。与 HF 组相比，咖啡摄入还减轻了脂肪组织炎症，减少了巨噬细胞浸润和 IL-6 水平（HF-CP：-337%，P <.05；HF-CT：-275%，P <.05）。HF-CP 和 HF-CT 组的肝脂肪变性和炎症均减轻。HF-CP 组与其他实验组相比，参与适应性产热和线粒体生物发生的基因（PPARγ、Prdm16、Pcg1α、β3-肾上腺素能受体、Ucp-1 和 Opa-1）的表达更为明显。与高脂肪饮食相关的预防性咖啡摄入可改善与肥胖及其合并症发展相关的代谢特征。
