脂组学研究贯叶连翘对小鼠化学性肝损伤的保护作用。
Lipidomic investigation of the protective effects of Polygonum perfoliatum against chemical liver injury in mice.
机构信息
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, Guizhou Province, China; Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang 550001, Guizhou Province, China; Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, Guizhou Province, China.
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, Guizhou Province, China; Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang 550001, Guizhou Province, China; Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, Guizhou Province, China.
出版信息
J Integr Med. 2023 May;21(3):289-301. doi: 10.1016/j.joim.2023.03.002. Epub 2023 Mar 9.
OBJECTIVE
Recent investigations have demonstrated that Polygonum perfoliatum L. can protect against chemical liver injury, but the mechanism behind its efficacy is still unclear. Therefore, we studied the pharmacological mechanism at work in P. perfoliatum protection against chemical liver injury.
METHODS
To evaluate the activity of P. perfoliatum against chemical liver injury, levels of alanine transaminase, lactic dehydrogenase, aspartate transaminase, superoxide dismutase, glutathione peroxidase and malondialdehyde were measured, alongside histological assessments of the liver, heart and kidney tissue. A nontargeted lipidomics strategy based on ultra-performance liquid chromatography quadrupole-orbitrap high-resolution mass spectrometry method was used to obtain the lipid profiles of mice with chemical liver injury and following treatment with P. perfoliatum; these profiles were used to understand the possible mechanisms behind P. perfoliatum's protective activity.
RESULTS
Lipidomic studies indicated that P. perfoliatum protected against chemical liver injury, and the results were consistent between histological and physiological analyses. By comparing the profiles of liver lipids in model and control mice, we found that the levels of 89 lipids were significantly changed. In animals receiving P. perfoliatum treatment, the levels of 8 lipids were significantly improved, relative to the model animals. The results showed that P. perfoliatum extract could effectively reverse the chemical liver injury and significantly improve the abnormal liver lipid metabolism of mice with chemical liver injury, especially glycerophospholipid metabolism.
CONCLUSION
Regulation of enzyme activity related to the glycerophospholipid metabolism pathway may be involved in the mechanism of P. perfoliatum's protection against liver injury. Please cite this article as: Peng L, Chen HG, Zhou X. Lipidomic investigation of the protective effects of Polygonum perfoliatum against chemical liver injury in mice. J Integr Med. 2023; 21(3): 289-301.
目的
最近的研究表明，贯叶连翘（Polygonum perfoliatum L.）可以预防化学性肝损伤，但它的疗效机制尚不清楚。因此，我们研究了贯叶连翘防治化学性肝损伤的药理机制。
方法
为评估贯叶连翘对化学性肝损伤的活性，测定了丙氨酸转氨酶、乳酸脱氢酶、天冬氨酸转氨酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和丙二醛的水平，并对肝、心和肾组织进行了组织学评估。采用基于超高效液相色谱-四极杆轨道阱高分辨质谱的非靶向脂质组学策略，获得化学性肝损伤小鼠及贯叶连翘治疗后小鼠的脂质谱；这些谱图用于了解贯叶连翘保护活性的可能机制。
结果
脂质组学研究表明，贯叶连翘对化学性肝损伤有保护作用，组织学和生理学分析结果一致。通过比较模型和对照小鼠的肝脏脂质谱，我们发现 89 种脂质的水平有显著变化。在接受贯叶连翘治疗的动物中，与模型动物相比，有 8 种脂质的水平显著改善。结果表明，贯叶连翘提取物能有效逆转化学性肝损伤，显著改善化学性肝损伤小鼠异常的肝脂质代谢，特别是甘油磷脂代谢。
结论
与甘油磷脂代谢途径相关的酶活性的调节可能参与了贯叶连翘防治肝损伤的机制。请引用本文作为：彭亮，陈华国，周霞。贯叶连翘防治化学性肝损伤的脂质组学研究。中国整合医学，2023；21（3）：289-301。
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