Robin J, Bernard J
Exp Biol. 1986;45(2):123-6.
A new method for the quantitative determination of carbohydrates on thin-layer chromatography has been devised. The method is based on charring the spots with sulphuric acid generated from interaction of sulphuryl chloride with water vapour, and quantification of carbohydrates by photodensitometry of the chromatograms. In contrast to current methods, this one does not require the use of several reagents and the charred plates can be stored without fading. The method is simple, sensitive and reproducible.
已设计出一种在薄层色谱上定量测定碳水化合物的新方法。该方法基于用由硫酰氯与水蒸气相互作用产生的硫酸使斑点炭化，并通过对色谱图进行光密度测定来定量碳水化合物。与现有方法相比，该方法不需要使用多种试剂，且炭化后的板可以保存而不褪色。该方法简单、灵敏且可重复。
