Srebreva L, Zlatanova J
FEBS Lett. 1986 May 5;200(1):123-7. doi: 10.1016/0014-5793(86)80523-3.
Antibodies were elicited against chromatographically purified histone H1 subfractions or against their complexes with RNA and their specificity studied by enzyme-linked immunosorbent assay. The results show that complexing of the pure protein with RNA does not lead to any significant increase in the antibody titer and results in obtaining antibodies predominantly against the common antigenic determinants present in the H1 histone class. On the other hand, using pure histone fractions for immunization gives rise to antibody populations reacting mainly with the subfraction-specific determinants on the histone molecule. In view of these results the literature data should be interpreted with caution.
针对经色谱纯化的组蛋白H1亚组分或其与RNA的复合物产生抗体，并通过酶联免疫吸附测定研究其特异性。结果表明，纯蛋白与RNA复合不会导致抗体效价显著增加，且主要产生针对H1组蛋白类别中存在的共同抗原决定簇的抗体。另一方面，使用纯组蛋白组分进行免疫会产生主要与组蛋白分子上亚组分特异性决定簇反应的抗体群体。鉴于这些结果，对文献数据的解读应谨慎。
