一种预防家猫梨形虫病的新型疫苗策略。

A Novel Vaccine Strategy to Prevent Cytauxzoonosis in Domestic Cats.

作者信息

Weerarathne Pabasara, Maker Rebekah, Huang Chaoqun, Taylor Brianne, Cowan Shannon R, Hyatt Julia, Tamil Selvan Miruthula, Shatnawi Shoroq, Thomas Jennifer E, Meinkoth James H, Scimeca Ruth, Birkenheuer Adam, Liu Lin, Reichard Mason V, Miller Craig A

机构信息

Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA.

Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA.

出版信息

Vaccines (Basel). 2023 Mar 2;11(3):573. doi: 10.3390/vaccines11030573.

Cytauxzoonosis is caused by (), a tick-borne parasite that causes severe disease in domestic cats in the United States. Currently, there is no vaccine to prevent this fatal disease, as traditional vaccine development strategies have been limited by the inability to culture this parasite in vitro. Here, we used a replication-defective human adenoviral vector (AdHu5) to deliver -specific immunogenic antigens and induce a cell-mediated and humoral immune response in cats. Cats ( = 6 per group) received either the vaccine or placebo in two doses, 4 weeks apart, followed by experimental challenge with at 5 weeks post-second dose. While the vaccine induced significant cell-mediated and humoral immune responses in immunized cats, it did not ultimately prevent infection with . However, immunization significantly delayed the onset of clinical signs and reduced febrility during infection. This AdHu5 vaccine platform shows promising results as a vaccination strategy against cytauxzoonosis.

嗜吞噬细胞无形体病由（嗜吞噬细胞无形体）引起，这是一种通过蜱传播的寄生虫，在美国会导致家猫患上严重疾病。目前，尚无预防这种致命疾病的疫苗，因为传统的疫苗开发策略受到无法在体外培养这种寄生虫的限制。在此，我们使用复制缺陷型人腺病毒载体（AdHu5）来递送特异性免疫原性抗原，并在猫体内诱导细胞介导和体液免疫反应。猫（每组 = 6只）分两剂接受疫苗或安慰剂，间隔4周，在第二剂接种后5周用（嗜吞噬细胞无形体）进行实验性攻毒。虽然疫苗在免疫的猫中诱导了显著的细胞介导和体液免疫反应，但它最终并未预防（嗜吞噬细胞无形体）感染。然而，免疫显著延迟了临床症状的出现，并在（嗜吞噬细胞无形体）感染期间降低了发热程度。这种AdHu5疫苗平台作为一种针对嗜吞噬细胞无形体病的疫苗接种策略显示出了有前景的结果。