Departamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Av. Professor Moraes Rego, S/N, Campus da UFPE, Cidade Universitária, Recife 50740-465, PE, Brazil.
Biotério de Criação, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Av. Professor Moraes Rego, S/N, Campus da UFPE, Cidade Universitária, Recife 50740-465, PE, Brazil.
Viruses. 2023 Mar 21;15(3):799. doi: 10.3390/v15030799.
Mayaro virus (MAYV) is transmitted by spp. mosquitoes and has been circulating in Amazon areas in the North and Central West regions of Brazil since the 1980s, with an increase in human case notifications in the last 10 years. MAYV introduction in urban areas is a public health concern as infections can cause severe symptoms similar to other alphaviruses. Studies with have demonstrated the potential vector competence of the species and the detection of MAYV in urban populations of mosquitoes. Considering the two most abundant urban mosquito species in Brazil, we investigated the dynamics of MAYV transmission by and in a mice model. Mosquito colonies were artificially fed with blood containing MAYV and infection (IR) and dissemination rates (DR) were evaluated. On the 7th day post-infection (dpi), IFNAR BL/6 mice were made available as a blood source to both mosquito species. After the appearance of clinical signs of infection, a second blood feeding was performed with a new group of non-infected mosquitoes. RT-qPCR and plaque assays were carried out with animal and mosquito tissues to determine IR and DR. For , we found an IR of 97.5-100% and a DR reached 100% in both 7 and 14 dpi. While IR and DR for was 13.1-14.81% and 60% to 80%, respectively. A total of 18 mice were used (test = 12 and control = 6) for and 12 (test = 8 and control = 4) for to evaluate the mosquito-mice transmission rate. All mice that were bitten by infected showed clinical signs of infection while all mice exposed to infected mosquitoes remained healthy. Viremia in the mice from group ranged from 2.5 × 10 to 5 × 10 PFU/mL. from the second blood feeding showed a 50% IR. Our study showed the applicability of an efficient model to complete arbovirus transmission cycle studies and suggests that the population evaluated is a competent vector for MAYV, while highlighting the vectorial capacity of and the possible introduction into urban areas. The mice model employed here is an important tool for arthropod-vector transmission studies with laboratory and field mosquito populations, as well as with other arboviruses.
玛雅罗病毒（MAYV）由 spp. 蚊子传播，自 20 世纪 80 年代以来一直在巴西北部和中西部地区的亚马逊地区流行，在过去 10 年中，人类病例报告有所增加。MAYV 在城市地区的传播引起了公共卫生关注，因为感染可能导致类似于其他甲病毒的严重症状。研究表明，这些物种具有潜在的媒介能力，并且在城市蚊子种群中检测到了 MAYV。考虑到巴西最丰富的两种城市蚊子物种，我们在小鼠模型中研究了 和 传播 MAYV 的动态。蚊子种群通过人工吸食含有 MAYV 的血液，并评估感染率（IR）和传播率（DR）。在感染后第 7 天（dpi），IFNAR BL/6 小鼠作为两种蚊子的血液来源。在出现感染的临床症状后，用一组新的非感染蚊子进行了第二次血液喂养。对动物和蚊子组织进行 RT-qPCR 和噬菌斑分析，以确定 IR 和 DR。对于 ，我们发现 IR 为 97.5-100%，7 和 14 dpi 时 DR 达到 100%。而 的 IR 为 13.1-14.81%，7 和 14 dpi 时 DR 为 60%-80%。总共使用了 18 只小鼠（实验组 12 只，对照组 6 只）用于 ，使用了 12 只小鼠（实验组 8 只，对照组 4 只）用于 ，以评估蚊子-小鼠传播率。所有被感染的 叮咬的小鼠均出现感染的临床症状，而所有接触感染 蚊子的小鼠均保持健康。来自 组的小鼠的病毒血症范围为 2.5×10 到 5×10 PFU/mL。来自第二次血液喂养的 显示出 50%的 IR。我们的研究表明，该模型适用于完成虫媒病毒传播周期研究，并表明评估的 种群是 MAYV 的有效媒介，同时强调了 和可能引入城市地区的可能性。这里使用的小鼠模型是研究实验室和野外蚊子种群以及其他虫媒病毒的节肢动物-媒介传播的重要工具。
