Sato Airi, Yew Joanne Y, Takahashi Aya
Department of Biological Sciences, Tokyo Metropolitan University, Minamiosawa, Hachioji, Japan.
Pacific Biosciences Research Center, University of Hawai'i at Mānoa, Honolulu, HI, United States.
bioRxiv. 2023 Mar 23:2023.03.20.533419. doi: 10.1101/2023.03.20.533419.
Oviposition site choice has a large impact on offspring performance. Unlike other vinegar flies that colonize decaying fruits, lay eggs into hard ripening fruits by using their enlarged and serrated ovipositors (oviscapts). This behavior has an advantage over other species by providing access to the host fruit earlier and avoiding competition. However, the larvae are not fully adapted to a low-protein diet, and the availability of intact healthy fruits is seasonally restricted. Thus, to investigate oviposition site preference for microbial growth in this species, we conducted an oviposition assay using single species of commensal acetic acid bacteria, and . The oviposition site preferences for media with or without bacterial growth were quantified in multiple strains of and its closely related species, and , and a typical fermenting-fruit consumer, . Our comparisons demonstrated a continuous degree of preference for sites with growth both within and across species, suggesting that the niche separation is notable but not complete. The preference for showed large variations among replicates and no clear differences between the strains. In addition, the lack of interspecific differences in feeding site preference for -containing media implies that the interspecific divergence in oviposition site preference occurred independently from the feeding site preference. Our oviposition assays measuring the preference of multiple strains from each fly species for acetic acid bacteria growth revealed intrinsic properties of shared resource usage among these fruit fly species.
产卵地点的选择对后代的表现有很大影响。与其他在腐烂果实上定殖的果蝇不同，[该果蝇]通过使用其扩大且有锯齿的产卵器（产卵鞘）将卵产在硬熟的果实中。这种行为比其他物种具有优势，因为它能更早地接触到宿主果实并避免竞争。然而，幼虫并未完全适应低蛋白饮食，而且完整健康果实的可获得性受季节限制。因此，为了研究该物种对微生物生长的产卵地点偏好，我们使用单一的共生醋酸杆菌物种进行了产卵试验。在[该果蝇]及其近缘物种以及典型的发酵果实消费者[另一果蝇物种]的多个菌株中，对有或没有细菌生长的培养基的产卵地点偏好进行了量化。我们的比较表明，在物种内部和物种之间，对有[细菌]生长的地点都存在持续程度的偏好，这表明生态位分离显著但并不完全。对[细菌]的偏好在重复实验中表现出很大差异，且菌株之间没有明显差异。此外，对含[细菌]培养基的取食地点偏好缺乏种间差异，这意味着产卵地点偏好的种间差异独立于取食地点偏好而发生。我们测量每个果蝇物种多个菌株对醋酸杆菌生长偏好的产卵试验揭示了这些果蝇物种之间共享资源利用的内在特性。
