Wang Dan, Song Jian, Lin Tuanrong, Yin Yuhe, Mu Junxiang, Liu Shuancheng, Wang Yaqin, Kong Dejuan, Zhang Zhicheng
College of Life Sciences and Technology Jining Normal University Ulanqab Inner Mongolia China.
Institute of Industrial Crops Shanxi Agricultural University Taiyuan Shanxi China.
Plant Direct. 2023 Mar 27;7(3):e491. doi: 10.1002/pld3.491. eCollection 2023 Mar.
Lipid transfer proteins (LTPs) are widely distributed in plants and play an important role in the response to stress. Potato ( L.) is sensitive to a lack of water, and drought stress is one of the limiting factors for its yield. Therefore, mining candidate functional genes for drought stress and creating new types of potato germplasm for drought resistance is an effective way to solve this problem. There are few reports on the family in potato. In this study, 39 members of the potato family were identified. They were located on seven chromosomes, and the amino acid sequences encoded ranged from 101 to 345 aa. All 39 family members contained introns and had exons that ranged from one to four. Conserved motif analysis of potato LTP transcription factors showed that 34 transcription factors contained Motif 2 and Motif 4, suggesting that they were conserved motifs of potato LTP. Compared with the genes of homologous crops, the potato and tomato ( L.) LTPs were the mostly closely related. The and genes were screened by quantitative reverse transcription PCR combined with potato transcriptome data to study their expression in tissues and the characteristics of their responses to drought stress. The results showed that and were upregulated in the roots, stems, and leaves after PEG 6000 stress. Taken together, our study provides comprehensive information on the potato LTP family that will help to develop a framework for further functional studies.
脂质转移蛋白（LTPs）广泛分布于植物中，在应对胁迫方面发挥着重要作用。马铃薯（Solanum tuberosum L.）对缺水敏感，干旱胁迫是其产量的限制因素之一。因此，挖掘干旱胁迫候选功能基因并培育新型抗旱马铃薯种质是解决这一问题的有效途径。关于马铃薯中该家族的报道较少。在本研究中，鉴定出了39个马铃薯LTP家族成员。它们位于7条染色体上，编码的氨基酸序列长度在101至345个氨基酸之间。所有39个家族成员都含有内含子，外显子数量从1到4个不等。马铃薯LTP转录因子的保守基序分析表明，34个转录因子含有基序2和基序4，这表明它们是马铃薯LTP的保守基序。与同源作物的LTP基因相比，马铃薯和番茄（Solanum lycopersicum L.）的LTPs亲缘关系最为密切。通过定量逆转录PCR结合马铃薯转录组数据筛选出StLTP1和StLTP2基因，以研究它们在组织中的表达以及对干旱胁迫的响应特征。结果表明，在PEG 6000胁迫后，StLTP1和StLTP2在根、茎和叶中上调表达。综上所述，我们的研究提供了关于马铃薯LTP家族的全面信息，这将有助于构建进一步功能研究的框架。
