Yang Shipeng, Zhu Haodong, Huang Liping, Zhang Guangnan, Wang Lihui, Jiang Xiaoting, Zhong Qiwen
Qinghai Key Laboratory of Vegetable Genetics and Physiology, Agriculture and Forestry Sciences Institute of Qinghai University, Qinghai University, Xining, P.R. China.
Qinghai University, Xining, P.R. China.
PeerJ. 2021 Mar 29;9:e10966. doi: 10.7717/peerj.10966. eCollection 2021.
(Pepino) is an increasingly popular solanaceous crop and is tolerant of drought conditions. In this study, 71 NAC transcription factor family genes of were selected to provide a theoretical basis for subsequent in-depth study of their regulatory roles in the response to biological and abiotic stresses, and were subjected to whole-genome analysis. The NAC sequences obtained by transcriptome sequencing were subjected to bioinformatics prediction and analysis. Three concentration gradient drought stresses were applied to the plants, and the target gene sequences were analyzed by qPCR to determine their expression under drought stress. The results showed that the NAC family contains 71 genes, 47 of which have conserved domains. The protein sequence length, molecular weight, hydrophilicity, aliphatic index and isoelectric point of these transcription factors were predicted and analyzed. Phylogenetic analysis showed that the NAC gene family is divided into seven subfamilies. Some NAC genes of are closely related to the NAC genes of Solanaceae crops such as tomato, pepper and potato. The seedlings of were grown under different gradients of drought stress conditions and qPCR was used to analyze the NAC expression in roots, stems, leaves and flowers. The results showed that 13 genes did not respond to drought stress while 58 NAC genes of that responded to drought stress had obvious tissue expression specificity. The overall expression levels in the root were found to be high. The number of genes at extremely significant expression levels was very large, with significant polarization. Seven NAC genes with significant responses were selected to analyze their expression trend in the different drought stress gradients. It was found that genes with the same expression trend also had the same or part of the same conserved domain. Seven SmNACs that may play an important role in drought stress were selected for NAC amino acid sequence alignment of crops. Four had strong similarity to other Solanaceae NAC amino acid sequences, and SmNAC has high homology with the . The NAC transcription factor family genes of showed strong structural conservation. Under drought stress, the expression of NAC transcription factor family genes of changed significantly, which actively responded to and participated in the regulation process of drought stress, thereby laying foundations for subsequent in-depth research of the specific functions of NAC transcription factor family genes of .
番荔枝是一种越来越受欢迎的茄科作物，耐旱。本研究选取了71个番荔枝NAC转录因子家族基因进行全基因组分析，为后续深入研究其在生物和非生物胁迫响应中的调控作用提供理论依据。对转录组测序获得的NAC序列进行生物信息学预测和分析。对植株施加三个浓度梯度的干旱胁迫，通过qPCR分析目标基因序列，以确定其在干旱胁迫下的表达。结果表明，番荔枝NAC家族包含71个基因，其中47个具有保守结构域。对这些转录因子的蛋白质序列长度、分子量、亲水性、脂肪族指数和等电点进行了预测和分析。系统发育分析表明，番荔枝NAC基因家族分为七个亚家族。番荔枝的一些NAC基因与番茄、辣椒和马铃薯等茄科作物的NAC基因密切相关。在不同梯度干旱胁迫条件下培养番荔枝幼苗，用qPCR分析根、茎、叶和花中NAC的表达。结果表明，13个基因对干旱胁迫无响应，而58个响应干旱胁迫的番荔枝NAC基因具有明显的组织表达特异性。发现根中的总体表达水平较高。极显著表达水平的基因数量非常多，存在显著的两极分化。选择7个响应显著的NAC基因分析其在不同干旱胁迫梯度下的表达趋势。发现具有相同表达趋势的基因也具有相同或部分相同的保守结构域。选择7个可能在干旱胁迫中起重要作用的番荔枝NAC进行茄科作物的NAC氨基酸序列比对。4个与其他茄科NAC氨基酸序列具有高度相似性，番荔枝NAC与（此处原文缺失相关内容）具有高度同源性。番荔枝的NAC转录因子家族基因显示出很强的结构保守性。在干旱胁迫下，番荔枝NAC转录因子家族基因的表达发生显著变化，积极响应并参与干旱胁迫的调控过程，从而为后续深入研究番荔枝NAC转录因子家族基因的具体功能奠定基础。
