College of Life Sciences, Jilin Agricultural University, Changchun 130118, China.
College of Agronomy, Jilin Agricultural University, Changchun 130118, China.
Int J Mol Sci. 2022 Oct 16;23(20):12378. doi: 10.3390/ijms232012378.
Soybean transcription factor GmNAC plays important roles in plant resistance to environmental stresses. In this study, was cloned in the drought tolerant soybean variety "Jiyu47", with the molecular properties of GmNAC3 characterized to establish its candidacy as a NAC transcription factor. The yeast self-activation experiments revealed the transcriptional activation activity of GmNAC3, which was localized in the nucleus by the subcellular localization analysis. The highest expression of was detected in roots in the podding stage of soybean, and in roots of soybean seedlings treated with 20% PEG6000 for 12 h, which was 16 times higher compared with the control. In the transgenic soybean hairy roots obtained by the -mediated method treated with 20% PEG6000 for 12 h, the activities of superoxide dismutase, peroxidase, and catalase and the content of proline were increased, the malondialdehyde content was decreased, and the expressions of stress resistance-related genes (i.e., , , , and ) were up-regulated. These expression patterns were confirmed by transgenic with the overexpression of . This study provided strong scientific evidence to support further investigation of the regulatory function of in plant drought resistance and the molecular mechanisms regulating the plant response to environmental stresses.
大豆转录因子 GmNAC 在植物抵抗环境胁迫中发挥重要作用。本研究在耐旱大豆品种“冀育 47”中克隆了 GmNAC3，对其分子特性进行了表征，确立了其作为 NAC 转录因子的候选地位。酵母自激活实验显示 GmNAC3 具有转录激活活性，亚细胞定位分析表明其定位于细胞核。在大豆结荚期的根中检测到 GmNAC3 的表达最高，在大豆幼苗用 20%PEG6000 处理 12 h 后，其表达量比对照高 16 倍。在通过农杆菌介导的方法获得的转基因大豆毛状根中，用 20%PEG6000 处理 12 h 后，超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性以及脯氨酸的含量增加，丙二醛的含量降低，与抗逆性相关的基因（即、、、和）的表达上调。这些表达模式通过过表达 得到了证实。本研究为进一步研究 GmNAC 在植物抗旱性中的调控功能以及调控植物对环境胁迫反应的分子机制提供了有力的科学依据。
