头皮针益智康疗法在百会（GV20）、四神聪（EX-HN1）、智三针、捏三针通过 Notch 信号通路改善脑瘫幼鼠的神经行为。
Scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway.
机构信息
Department of Acupuncture and Message, Hebei University of Chinese Medicine, Shijiazhuang 050091, China.
Department of Child Rehabilitation, the First Affiliated Hospital of Hebei University of Chinese Medicine, Shijiazhuang 050013, China.
出版信息
J Tradit Chin Med. 2023 Apr;43(2):337-342. doi: 10.19852/j.cnki.jtcm.20221206.002.
OBJECTIVE
To investigate the efficacy of scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen, on neurobehavior in young rats with cerebral palsy based on Notch signaling pathway.
METHODS
Thirty 7-day-old rats were randomly divided into sham, model and acupuncture, 10 rats in each group. The cerebral palsy model was established by the accepted modeling method, the acupuncture group selected "Baihui (GV20)", "Sishencong (EX-HN1)", "Zhisanzhen" and "Niesanzhen" for intervention 24 h after the model was made. The body masses were recorded before and after the treatment, respectively. After the intervention, the rats were subjected to suspension experiment, slope experiment, tactile stimulation experiment and Morris water maze experiment. After the end of the experiment, the morphological changes of hippocampal histology were observed by hematoxylin-eosin (HE) staining under light microscope, and the expression of Notch1, Notch3 and Hes5 were detected by Western blot and quantitative real-time polymerase chain reaction (PCR).
RESULTS
The changes in body mass of the rats in each group were different; in behavioral experiments, compared with the sham, the suspension time of the model was shortened, the slope experiment, tactile stimulation experiment, and escape latency time were prolonged, and the number of platform crossing was reduced in the model, compared with the model, the suspension time of the acupuncture was prolonged, the slope experiment, tactile stimulation experiment, and escape latency time were shortened, and the number of platform crossing times was increased; HE staining showed severe hippocampal damage in the model and reduced hippocampal damage in the acupuncture. Western Blot and real-time fluorescence quantitative PCR showed that the expression of Notch1, Notch3 and Hes5 were increased in the model and the expression of Notch1, Notch3, Hes5 in acupuncture were decreased.
CONCLUSIONS
Scalp acupuncture Yikang therapy may improve neurobehavior and reduce brain injury in rats with cerebral palsy by downregulating the expression of Notch1, Notch3, and Hes5.
目的
基于 Notch 信号通路探讨头针益智疗法对百会（GV20）、四神聪（EX-HN1）、智三针、捻转针法治疗脑瘫幼鼠神经行为的影响。
方法
将 30 只 7 日龄大鼠随机分为假手术组、模型组、针刺组，每组 10 只。采用经典造模方法建立脑瘫模型，针刺组在造模后 24 h 选取“百会（GV20）”“四神聪（EX-HN1）”“智三针”“捻转针法”进行干预。分别于治疗前后记录大鼠体质量。干预后行悬吊实验、斜坡实验、触须刺激实验、Morris 水迷宫实验，实验结束后行海马组织苏木精-伊红（HE）染色光镜下形态学观察，Western blot 和实时荧光定量聚合酶链反应（PCR）检测 Notch1、Notch3、Hes5 的表达。
结果
各组大鼠体质量变化不同；行为学实验中，与假手术组比较，模型组的悬吊时间缩短，斜坡实验、触须刺激实验、逃避潜伏期时间延长，平台穿越次数减少；与模型组比较，针刺组的悬吊时间延长，斜坡实验、触须刺激实验、逃避潜伏期时间缩短，平台穿越次数增加；HE 染色显示模型组海马组织损伤严重，针刺组海马组织损伤减轻。Western blot 和实时荧光定量 PCR 显示模型组 Notch1、Notch3、Hes5 表达升高，针刺组 Notch1、Notch3、Hes5 表达降低。
结论
头针益智疗法可能通过下调 Notch1、Notch3、Hes5 的表达，改善脑瘫幼鼠的神经行为，减轻脑损伤。
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