Tang Xiaoling, Chen Jingxiang, Liu Zhiqiang, Zheng Yuguo
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Mar 25;39(3):993-1008. doi: 10.13345/j.cjb.220781.
The development of synthetic biology has greatly promoted the construction of microbial cell factories, providing an important strategy for green and efficient chemical production. However, the bottleneck of poor tolerance to harsh industrial environments has become the key factor hampering the productivity of microbial cells. Adaptive evolution is an important method to domesticate microorganisms for a certain period by applying targeted selection pressure to obtain desired phenotypic or physiological properties that are adapted to a specific environment. Recently, with the development of technologies such as microfluidics, biosensors, and omics analysis, adaptive evolution has laid the foundation for efficient productivity of microbial cell factories. Herein, we discuss the key technologies of adaptive evolution and their important applications in improvement of environmental tolerance and production efficiency of microbial cell factories. Moreover, we looked forward to the prospects of adaptive evolution to realize industrial production by microbial cell factories.
合成生物学的发展极大地推动了微生物细胞工厂的构建，为绿色高效的化学品生产提供了重要策略。然而，对恶劣工业环境耐受性差这一瓶颈已成为阻碍微生物细胞生产力的关键因素。适应性进化是一种重要方法，通过施加定向选择压力对微生物进行一定时期的驯化，以获得适应特定环境的理想表型或生理特性。近年来，随着微流控、生物传感器和组学分析等技术的发展，适应性进化为微生物细胞工厂的高效生产力奠定了基础。在此，我们讨论适应性进化的关键技术及其在提高微生物细胞工厂环境耐受性和生产效率方面的重要应用。此外，我们展望了适应性进化实现微生物细胞工厂工业化生产的前景。
