Zhao Yangyang, Liu Yinshuang, Niu Hongjin, Jia Zhenhua, Li Zaixing, Chen Xiaobo, Huang Yali
School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, Hebei, China.
Institute of Biology, Hebei Academy of Sciences, Shijiazhuang 050081, Hebei, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Mar 25;39(3):1175-1187. doi: 10.13345/j.cjb.220568.
In order to determine the changes of bacterial community structure and function in the early, middle and late stage of aerobic composting of chicken manure, high-throughput sequencing and bioinformatics methods were used to determine and analyze the 16S rRNA sequence of samples at different stages of composting. Wayne analysis showed that most of the bacterial OTUs in the three composting stages were the same, and only about 10% of the operational taxonomic units (OTUs) showed stage specificity. The diversity indexes including Ace, Chao1 and Simpson showed a trend of increasing at first, followed by decreasing. However, there was no significant difference among different composting stages ( < 0.05). The dominant bacteria groups in three composting stages were analyzed at the phylum and genus levels. The dominant bacteria phyla at three composting stages were the same, but the abundances were different. LEfSe (line discriminant analysis (LDA) effect size) method was used to analyze the bacterial biological markers with statistical differences among three stages of composting. From the phylum to genus level, there were 49 markers with significant differences among different groups. The markers included 12 species, 13 genera, 12 families, 8 orders, 1 boundary, and 1 phylum. The most biomarkers were detected at early stage while the least biomarkers were detected at late stage. The microbial diversity was analyzed at the functional pathway level. The function diversity was the highest in the early stage of composting. Following the composting, the microbial function was enriched relatively while the diversity decreased. This study provides theoretical support and technical guidance for the regulation of livestock manure aerobic composting process.
为了确定鸡粪好氧堆肥早、中、后期细菌群落结构和功能的变化，采用高通量测序和生物信息学方法对堆肥不同阶段样品的16S rRNA序列进行测定和分析。韦恩分析表明，三个堆肥阶段的大多数细菌OTU相同，只有约10%的可操作分类单元（OTU）表现出阶段特异性。包括Ace、Chao1和Simpson在内的多样性指数呈现出先增加后降低的趋势。然而，不同堆肥阶段之间没有显著差异（<0.05）。在门和属水平上分析了三个堆肥阶段的优势细菌群。三个堆肥阶段的优势细菌门相同，但丰度不同。采用线性判别分析效应大小（LEfSe）方法分析堆肥三个阶段具有统计学差异的细菌生物标志物。从门到属水平，不同组之间有49个具有显著差异的标志物。这些标志物包括12个种、13个属、12个科、8个目、1个界和1个门。在早期检测到的生物标志物最多，而在后期检测到的生物标志物最少。在功能途径水平上分析了微生物多样性。堆肥早期功能多样性最高。随着堆肥进行，微生物功能相对富集而多样性降低。本研究为畜禽粪便好氧堆肥过程的调控提供了理论支持和技术指导。
