Moore S K, James E, James P M, Fareed G
Gene. 1978 Nov;4(3):261-78. doi: 10.1016/0378-1119(78)90022-7.
A 1650 base pair (BP) fragment carrying the entire argF structural gene with its associated control regions was isolated from an EcoRI/BamHI digest of phi80argFilambda cI857 DNA. This segment was cloned using the EcoRI and BamHI cleavage sites in the plasmid pBR322. A preliminary restriction map of the argF region was prepared. RNA polymerase binding studies indicated that the argF promoter is located approx. 30 base pairs from the EcoRI terminus of the cloned DNA segment.
从噬菌体phi80argFilambda cI857 DNA的EcoRI/BamHI酶切片段中分离出一个1650碱基对（bp）的片段，该片段携带完整的argF结构基因及其相关的控制区域。使用质粒pBR322中的EcoRI和BamHI切割位点对该片段进行克隆。制备了argF区域的初步限制性图谱。RNA聚合酶结合研究表明，argF启动子位于克隆DNA片段的EcoRI末端约30个碱基对处。
