Sancar A, Rupp W D
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3144-8. doi: 10.1073/pnas.76.7.3144.
We have cloned the recA gene of Echerichia coli K12 and some of its restriction fragments on the plasmid cloning vehicle pBR322. The recA gene was mapped with regard to the restriction sites of EcoRI, BamHI, Pst I, Hha I, Hae III, HinfI, and Taq I restriction endonucleases. The recA promoter was localized by the binding of RNA polymerase to restriction fragments. The initiation point of transcription of recA mRNA and the direction of transcription were determined from in vitro transcription of recA gene fragments and from analysis of the polypeptides made in maxicells that contain plasmids carrying only part of the recA gene.
我们已将大肠杆菌K12的recA基因及其一些限制性片段克隆到质粒克隆载体pBR322上。针对EcoRI、BamHI、Pst I、Hha I、Hae III、HinfI和Taq I限制性内切酶的限制性位点对recA基因进行了定位。通过RNA聚合酶与限制性片段的结合来定位recA启动子。recA mRNA的转录起始点和转录方向是通过recA基因片段的体外转录以及对含有仅携带部分recA基因的质粒的大细胞中产生的多肽的分析来确定的。
