Hu M, Deonier R C
Mol Gen Genet. 1981;181(2):222-9. doi: 10.1007/BF00268430.
Two directly-repeated IS1 elements have been mapped on the Escherichia coli K-12 chromosome at positions 23.2 kb and 34.5 kb counterclockwise of the IS3 element alpha3beta3 by using F-prime plasmids (including the F lac- proAB+ plasmid F128) that carry different portions of the bacterial chromosome in the purE to proA region. Mapping was accomplished in part by construction of EcoRI, BamHI, and BglII restriction enzyme cleavage maps. Electron microscope heteroduplex and hybridization studies indicate that the chromosomal region flanked by these IS1 elements is completely homologous to the IS1-argF-IS1 region (Tn2901) on the P1argF5 transducing phage (York and Stodolsky, 1981), which suggests that the argF gene region in the usual E. coli K-12 strains has a transposon-like structure.
利用携带细菌染色体purE到proA区域不同部分的F'质粒（包括F lac - proAB + 质粒F128），已将两个同向重复的IS1元件定位在大肠杆菌K - 12染色体上，位于IS3元件α3β3逆时针方向23.2 kb和34.5 kb处。定位部分是通过构建EcoRI、BamHI和BglII限制酶切割图谱完成的。电子显微镜异源双链体和杂交研究表明，这些IS1元件侧翼的染色体区域与P1argF5转导噬菌体上的IS1 - argF - IS1区域（Tn2901）完全同源（York和Stodolsky，1981），这表明普通大肠杆菌K - 12菌株中的argF基因区域具有转座子样结构。
