希腊葡萄病毒 L 的鉴定和遗传多样性。

Identification and genetic diversity of grapevine virus L in Greece.

机构信息

Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.

Laboratory of Virology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute, 8 St. Delta str., 14561, Kifissia, Greece.

出版信息

Arch Virol. 2023 Mar 30;168(4):127. doi: 10.1007/s00705-023-05756-z.

DOI:10.1007/s00705-023-05756-z

PMID:36995397

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10063476/

Abstract

In this study, grapevine virus L (GVL) was identified for the first time in Greece through the application of high-throughput sequencing of total RNA from grapevine samples. Further investigation of the prevalence of GVL in Greek vineyards by RT-PCR revealed its presence in 5.5% (31/560) of the tested samples, which originated from six viticultural areas of the country. Comparative sequence analysis based on the CP gene revealed a high degree of genetic variability among GVL isolates, while phylogenetic analysis grouped the Greek isolates in three of the five phylogroups formed, with most of them being classified in phylogroup I.

摘要

在这项研究中，通过对葡萄样本总 RNA 进行高通量测序，首次在希腊鉴定出葡萄病毒 L（GVL）。通过 RT-PCR 进一步调查 GVL 在希腊葡萄园的流行情况，发现其存在于 5.5%（31/560）的测试样本中，这些样本来自该国六个葡萄种植区。基于 CP 基因的比较序列分析显示，GVL 分离株之间存在高度遗传变异性，而系统发育分析将希腊分离株分为五个系统发育群中的三个群，其中大多数被归类为系统发育群 I。