对葡萄品种白比诺中葡萄病毒 L 的遗传变异株进行全基因组序列分析。
Complete genome sequence analysis of a genetic variant of grapevine virus L from the grapevine cultivar Blanc du Bois.
机构信息
Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX, 78596, USA.
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77843, USA.
出版信息
Arch Virol. 2020 Aug;165(8):1905-1909. doi: 10.1007/s00705-020-04682-8. Epub 2020 May 30.
The complete genome sequences of two grapevine virus L (GVL) isolates collected from the wine grape cultivar Blanc du Bois (Vitis spp.: 'Florida D 6-148'×'Cardinal') in Texas were determined. The two genome sequences (excluding the polyA tail) were each 7594 nucleotide long and 99.7% identical to each other, but they shared only ~74% identity with those of previously published GVL isolates. Further analysis showed that the two Texas GVL isolates also diverged significantly from previously published isolates of the virus in each of the five ORFs at both the nucleotide and amino acid level, indicating that they represent a new phylogroup of this virus.
从德克萨斯州的酿酒葡萄品种 Blanc du Bois（Vitis spp.：'Florida D 6-148'×'Cardinal'）中分离到的两个葡萄卷叶病毒 L（GVL）分离物的完整基因组序列已被确定。两个基因组序列（不包括 polyA 尾）的长度均为 7594 个核苷酸，彼此之间的相似度为 99.7%，但与先前发表的 GVL 分离物的相似度仅约为 74%。进一步的分析表明，这两个德克萨斯州的 GVL 分离物在 5 个 ORF 中的核苷酸和氨基酸水平上与先前发表的病毒分离物也存在显著差异，表明它们代表了该病毒的一个新的系统发育群。
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