Buch Thorsten, Jerchow Boris, Zevnik Branko
Institute of Laboratory Animal Science, University of Zurich, Zurich, Switzerland.
Novartis Institute for Biomedical Research (NIBR), Novartis Pharma AG, Basel, Switzerland.
Methods Mol Biol. 2023;2631:33-51. doi: 10.1007/978-1-0716-2990-1_2.
The principles of the 3Rs (replace, reduce, refine), as originally published by Russell and Burch, are internationally acclaimed guidelines for meeting ethical and welfare standards in animal experimentation. Genome manipulation is a standard technique in biomedical research and beyond. The goal of this chapter is to give practical advice on the implementation of the 3Rs in laboratories generating genetically modified rodents. We cover 3R aspects from the planning phase through operations of the transgenic unit to the final genome-manipulated animals. The focus of our chapter is on an easy-to-use, concise protocol that is close to a checklist. While we focus on mice, the proposed methodological concepts can be easily adapted for the manipulation of other sentient animals.
罗素和伯奇最初提出的3R原则（替代、减少、优化）是国际公认的动物实验伦理和福利标准指南。基因组操作是生物医学研究及其他领域的一项标准技术。本章的目的是就转基因啮齿动物生成实验室中实施3R原则提供实用建议。我们涵盖了从规划阶段到转基因单元操作再到最终基因组操作动物的3R各个方面。本章重点是一个易于使用、简洁的方案，类似于一份清单。虽然我们重点关注小鼠，但所提出的方法概念可轻松适用于其他有感知能力动物的操作。
