Department of Pharmaceutical Engineering, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran; Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
Department of Pharmaceutical Engineering, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
Int J Biol Macromol. 2023 Jun 1;239:124257. doi: 10.1016/j.ijbiomac.2023.124257. Epub 2023 Mar 28.
In this work, the antibacterial activity of Satureja Khuzestanica essential oil nanoemulsions improved by employing chitosan (ch/SKEO NE) against E. coli bacterium. The optimum ch/SKEO NE with mean droplet size of 68 nm was attained at 1.97, 1.23, and 0.10%w/w of surfactant, essential oil and chitosan, using Response Surface Methodology (RSM). Applying microfluidic platform, the ch/SKEO NE resulted in improved antibacterial activity owing to the modification of surface properties. The nanoemulsion samples showed a significant rupturing effect on the E. coli bacterial cell membrane which resulted in a rapid release of cellular contents. This action was remarkably intensified by executing microfluidic chip in parallel to the conventional method. Having treated the bacteria in the microfluidic chip for 5 min with a 8 μg/mL concentration of ch/SKEO NE, the bacterial integrity disrupted quickly, and the activity was totally lost in a 10-min period at 50 μglmL, while it took 5 h for a complete inhibition in the conventional method using the same concentration of ch/SKEO NE. It can be concluded that nanoemulsification of EOs using chitosan coating can intensify the interaction of nanodroplets with the bacterial membrane, especially within the microfluidic chips which provides high contact surface area.
在这项工作中，通过使用壳聚糖（ch/SKEO NE）对香荆芥精油纳米乳液进行改良，提高了其对大肠杆菌的抗菌活性。采用响应面法（RSM），在表面活性剂、精油和壳聚糖的浓度分别为 1.97%、1.23%和 0.10%w/w 时，得到平均粒径为 68nm 的最优 ch/SKEO NE。应用微流控平台，由于表面性质的改变，ch/SKEO NE 表现出了更好的抗菌活性。纳米乳液样品对大肠杆菌细胞膜表现出显著的破裂效应，导致细胞内容物迅速释放。通过在微流控芯片中与传统方法并行执行，这种作用得到了显著增强。在微流控芯片中以 8μg/mL 的 ch/SKEO NE 浓度处理细菌 5min 后，细菌完整性迅速被破坏，在 50μglmL 下 10min 内活性完全丧失，而在相同浓度的 ch/SKEO NE 下，使用传统方法则需要 5h 才能完全抑制。可以得出结论，使用壳聚糖涂层对精油进行纳米乳化可以增强纳米液滴与细菌膜的相互作用，特别是在微流控芯片中，它提供了高的接触表面积。
