Department of General Dental Practice, College of Dentistry, Kuwait University, Jabryia, Kuwait.
Department of Biological Sciences, College of Dentistry, Kuwait University, Kuwait.
J Dent. 2023 Jun;133:104486. doi: 10.1016/j.jdent.2023.104486. Epub 2023 Mar 28.
The aim of this study was to compare the antibacterial effect of different fluoride-containing and bioactive restorative materials, and their effect on the expression of specific biofilm-associated genes and therefore the caries process.
The restorative materials utilized in this study included: 1. Filtek Z250, 2. Fuji II LC, 3. Beautifil II, 4. ACTIVA, and 5. Biodentine. For each material, disc-shaped specimens were prepared. The inhibitory effects against Streptococcus mutans, Lactobacillus acidophilus, and Leptotrichia shahii were tested. After incubation for 24 h and 1 week, colony-forming units (CFUs) were enumerated. From the plates dedicated for biomass quantification and RNA purification, the target glucosyltransferase B (gtfB) and glucan-binding protein B (gbpB) genes were chosen for S. mutans. For L. acidophilus, a gene involved in exopolysaccharide synthesis (epsB) was chosen.
Except for Filtek Z250, all four materials showed statistically significant inhibitory effects on the biofilms of all three species. When biofilms were grown in the presence of the same four materials, the expression of S. mutans gtfB and gbpB genes, was significantly reduced. For L. acidophilus, the decrease in the expression of gtfB gene in the presence of ACTIVA was the highest change seen. The epsB gene expression also decreased. Compared to fluoride-releasing materials, bioactive materials had more inhibitory effect against L. acidophilus, both at 24 h and 1 week.
Both fluoride releasing and bioactive materials exhibited a significant inhibitory effect on the biofilm growth. The expression of the targeted biofilm-associated genes was downregulated by both material groups.
The findings from this study give insight into the antibacterial effect of fluoride-containing and bioactive materials which would help to reduce the chances for secondary caries and therefore increase the lifetime of dental restorations placed for patients.
本研究旨在比较不同含氟和生物活性修复材料的抗菌效果，及其对特定生物膜相关基因表达的影响，进而对龋齿进程的影响。
本研究中使用的修复材料包括：1. Filtek Z250，2. Fuji II LC，3. Beautifil II，4. ACTIVA，和 5. Biodentine。为每种材料制备圆盘状标本。测试其对变异链球菌、嗜酸乳杆菌和纤毛菌的抑制作用。孵育 24 小时和 1 周后，计数菌落形成单位（CFU）。从专门用于生物量定量和 RNA 纯化的平板中，选择目标变异链球菌葡糖基转移酶 B（gtfB）和葡聚糖结合蛋白 B（gbpB）基因。对于嗜酸乳杆菌，选择参与胞外多糖合成（epsB）的基因。
除 Filtek Z250 外，其余四种材料对三种细菌生物膜均表现出统计学显著的抑制作用。当生物膜在相同的四种材料存在下生长时，变异链球菌 gtfB 和 gbpB 基因的表达显著降低。对于嗜酸乳杆菌，在 ACTIVA 存在下，gtfB 基因的表达降低幅度最大。epsB 基因的表达也降低了。与释氟材料相比，生物活性材料对嗜酸乳杆菌的抑制作用更强，在 24 小时和 1 周时均如此。
释氟和生物活性材料均对生物膜生长表现出显著的抑制作用。两组材料均下调了靶向生物膜相关基因的表达。
本研究的结果深入了解了含氟和生物活性材料的抗菌效果，有助于降低继发龋的发生几率，从而延长为患者放置的牙体修复体的使用寿命。
