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EPB41L4A-AS1 和 UNC5B-AS1 在骨肉瘤中具有诊断和预后意义。

EPB41L4A-AS1 and UNC5B-AS1 have diagnostic and prognostic significance in osteosarcoma.

机构信息

Shanghai Baoshan Center for Disease Control and Prevention, Shanghai, 201901, China.

Clinical Research Center, Shanghai Baoshan Luodian Hospital, No. 121 Luoxi Road, Baoshan District, Shanghai, 201908, China.

出版信息

J Orthop Surg Res. 2023 Mar 30;18(1):261. doi: 10.1186/s13018-023-03754-0.

DOI:10.1186/s13018-023-03754-0
PMID:36998043
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10064547/
Abstract

BACKGROUND

Deregulation of lncRNAs has been observed in human osteosarcoma. This study explored the diagnostic and prognostic significance of EPB41L4A-AS1 and UNC5B-AS1 in osteosarcoma.

METHODS

Relative levels of EPB41L4A-AS1 and UNC5B-AS1 were detected in osteosarcoma tissue samples and cells. The ability to distinguish osteosarcoma from health was assessed by receiver operating characteristic (ROC) curve construction. Kaplan-Meier (K-M) and Cox proportional-hazards analyses were performed for prognosis factors. The bioinformatics approach was used to identify targeting miRNA for EPB41L4A-AS1 and UNC5B-AS1. Kaplan-Meier survival curves and Whitney Mann U tests were conducted for validating the statistical significance. In cell culture experiments, the influence of EPB41L4A-AS1 and UNC5B-AS1 on proliferation, migration, and invasion of the osteosarcoma cell line was examined by CCK-8 and Transwell assays.

RESULTS

Levels of EPB41L4A-AS1 and UNC5B-AS1 were upregulated in osteosarcoma patients and cells compared with the healthy participants and normal cell lines. EPB41L4A-AS1 and UNC5B-AS1 have a potent ability to distinguish the patients with osteosarcoma from the health. EPB41L4A-AS1 and UNC5B-AS1 levels correlated with SSS stage. Patients with high levels of EPB41L4A-AS1 and UNC5B-AS1 had significantly shorter survival times. EPB41L4A-AS1 and UNC5B-AS1 were independent prognostic indexes for overall survival. miR-1306-5p was a common target for EPB41L4A-AS1 and UNC5B-AS1. A propulsive impact on cell proliferation, migration, and invasion by EPB41L4A-AS1 and UNC5B-AS1 was observed, but can be rescued by miR-1306-5p.

CONCLUSIONS

It was concluded that upregulations of EPB41L4A-AS1 and UNC5B-AS1 expression were diagnostic and prognostic biomarkers for human osteosarcoma. EPB41L4A-AS1 and UNC5B-AS1 contribute to the biological behavior of osteosarcoma via miR-1306-5p.

摘要

背景

长链非编码 RNA 的失调已在人类骨肉瘤中观察到。本研究探讨了 EPB41L4A-AS1 和 UNC5B-AS1 在骨肉瘤中的诊断和预后意义。

方法

检测骨肉瘤组织样本和细胞中 EPB41L4A-AS1 和 UNC5B-AS1 的相对水平。通过构建接收器工作特征（ROC）曲线评估区分骨肉瘤与健康的能力。进行 Kaplan-Meier（K-M）和 Cox 比例风险分析以确定预后因素。采用生物信息学方法鉴定 EPB41L4A-AS1 和 UNC5B-AS1 的靶向 miRNA。进行 Kaplan-Meier 生存曲线和 Whitney Mann U 检验以验证统计意义。在细胞培养实验中，通过 CCK-8 和 Transwell 测定检查 EPB41L4A-AS1 和 UNC5B-AS1 对骨肉瘤细胞系增殖、迁移和侵袭的影响。

结果

与健康参与者和正常细胞系相比，骨肉瘤患者和细胞中 EPB41L4A-AS1 和 UNC5B-AS1 的水平上调。EPB41L4A-AS1 和 UNC5B-AS1 具有区分骨肉瘤患者与健康人群的强大能力。EPB41L4A-AS1 和 UNC5B-AS1 水平与 SSS 分期相关。EPB41L4A-AS1 和 UNC5B-AS1 水平高的患者生存时间明显缩短。EPB41L4A-AS1 和 UNC5B-AS1 是总生存期的独立预后指标。miR-1306-5p 是 EPB41L4A-AS1 和 UNC5B-AS1 的共同靶标。EPB41L4A-AS1 和 UNC5B-AS1 观察到对细胞增殖、迁移和侵袭的推进作用，但可被 miR-1306-5p 挽救。

结论

上调 EPB41L4A-AS1 和 UNC5B-AS1 的表达是人类骨肉瘤的诊断和预后生物标志物。EPB41L4A-AS1 和 UNC5B-AS1 通过 miR-1306-5p 促进骨肉瘤的生物学行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/5dd9a9cdc2d8/13018_2023_3754_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/8117ec5404be/13018_2023_3754_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/d168e9cd6eff/13018_2023_3754_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/5dd9a9cdc2d8/13018_2023_3754_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/8117ec5404be/13018_2023_3754_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/83de6871eeb1/13018_2023_3754_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/0c34723a6ca4/13018_2023_3754_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/d168e9cd6eff/13018_2023_3754_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0451/10064547/5dd9a9cdc2d8/13018_2023_3754_Fig5_HTML.jpg

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