Fang Qingjian, Wu Qingjuan, Huang Huiqin, Hu Yonghua, Li Chenghua, Mo Kunlian
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211, PR China.
Zhanjiang Experimental Station, CATAS, Zhanjiang, 524013, PR China.
Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005783.
A Gram-stain-positive, non-motile, rod-shaped, facultatively anaerobic bacterium, designated as IB182487, was isolated from a seashore sand sample collected from Zhaoshu Island, PR China. Strain IB182487 grew at pH 6.0-10.0 (optimum, pH 8.0), 4-45 °C (optimum, 25-30 °C) and with 0-17 % (w/v) NaCl (optimum, 2-10 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IB182487 belonged to the genus and was closely related to SMC 4352-2, (96.6 %), LMG 22858 (96.5 %), DSM 17723 (96.3 %) and DSM 25387 (96.1 %). Strain IB182487 had -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan and contained menaquinone MK-7 as the predominant isoprenoid quinone. Its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and three unidentified glycolipids. The major cellular fatty acids of strain IB182487 were iso-C and anteiso-C. The whole genome average nucleotide identity and digital DNA-DNA hybridization analysis between the isolate and its closely related type strains demonstrated that the strain significantly differed from other species. The genomic DNA G+C content of strain IB182487 was 37.4 mol%. On the basis of phenotypic and chemotaxonomic properties, phylogenetic relatedness as well as genomic characteristics, strain IB182487 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is IB182487 (=MCCC 1K04629=JCM 34523).
从中国山东省赵树岛采集的海滨砂样中分离出一株革兰氏阳性、无运动性、杆状、兼性厌氧细菌，命名为IB182487。菌株IB182487在pH 6.0 - 10.0（最适pH 8.0）、4 - 45°C（最适25 - 30°C）和0 - 17%（w/v）NaCl（最适2 - 10%）条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株IB182487属于该属，与[具体菌株1] SMC 4352 - 2（96.6%）、[具体菌株2] LMG 22858（96.5%）、[具体菌株3] DSM 17723（96.3%）和[具体菌株4] DSM 25387（96.1%）密切相关。菌株IB182487在细胞壁肽聚糖中的诊断性二氨基酸为[具体二氨基酸名称]，主要类异戊二烯醌为甲基萘醌MK - 7。其极性脂质由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、两种未鉴定的磷脂和三种未鉴定的糖脂组成。菌株IB182487的主要细胞脂肪酸为异-C[具体碳数]和anteiso-C[具体碳数]。该分离株与其密切相关的模式菌株之间的全基因组平均核苷酸同一性和数字DNA - DNA杂交分析表明，该菌株与其他[属名]物种有显著差异。菌株IB182487的基因组DNA G + C含量为37.4 mol%。基于表型、化学分类学特性、系统发育相关性以及基因组特征，菌株IB182487代表了[属名]属的一个新物种，为此提出[新物种名称] sp. nov.。[属名]的模式菌株为IB182487（= MCCC 1K04629 = JCM
